Addgene

We narrowed to 35,256 results for: lat

Showing: 11341 - 11360 of 35256 results

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Adeno-miR-23b

Plasmid

#112538

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-23b (MIR23B Human)

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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GP821

Plasmid

#164552

Purpose

Expresses Smo-FLAG K434R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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1026L=U6-3-gRNA#1[Pol-γ35]

Plasmid

#159674

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#1[Pol-γ35]

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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1026M=U6-3-gRNA#2[Pol-γ35]

Plasmid

#159675

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#2[Pol-γ35]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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GP823

Plasmid

#164554

Purpose

Expresses Smo-FLAG K448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP822

Plasmid

#164553

Purpose

Expresses Smo-FLAG K444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP806

Plasmid

#164534

Purpose

Expresses Smo noK-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-2

Plasmid

#164859

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-3

Plasmid

#164860

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-3

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-3

Plasmid

#164867

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-3

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2080

Plasmid

#155160

Purpose

Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF_L266A

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2081

Plasmid

#155161

Purpose

Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaF(Y261A_L265A_L266A)

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1305

Plasmid

#155143

Purpose

Expresses MlaFB, His tag on MlaF N-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(6xHis-TEV-MlaF)-MlaB

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1306

Plasmid

#155144

Purpose

Expresses MlaFB, His tag on MlaF C-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaF-6xHis)-MlaB

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1307

Plasmid

#155145

Purpose

Expresses MlaFB, His tag on MlaB N-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1308

Plasmid

#155146

Purpose

Expresses MlaFB, His tag on MlaB C-term

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(MlaB-6xHis)

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1833

Plasmid

#155149

Purpose

Expresses MlaF(1-250)-GCN4

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-250)-GCN4_Dimerization_Domain

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1957

Plasmid

#155151

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

added Streptavidin tag on MlaF N-terminus

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2074

Plasmid

#155155

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Use

Tags

Expression

Mutation

MlaB_T52E

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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