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Plasmid#103347PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-210-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-210-5p target (MIR210 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133a-3p
Plasmid#103221PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 CA
Plasmid#44830DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationQ70L (constitutive active)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Lnk-SH2
Plasmid#46451DepositorInsertLnk (Sh2b3 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 325-466PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(TPF)
Plasmid#15741DepositorInsertJnk1a1(APY) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationTyr-192PhePromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-142-5p
Plasmid#103241PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-142-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-142-5p target (MIR142 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7f-5p
Plasmid#103156PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7f-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(C)-SH2
Plasmid#46519DepositorInsertSyk(C) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 149-263PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-519c-3p
Plasmid#103608PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-519c-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-519c-3p target (MIR519C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-520c-3p
Plasmid#103610PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-520c-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-520c-3p target (MIR520C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-143-5p
Plasmid#103243PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-143-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-143-5p target (MIR143 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Vav1-SH2
Plasmid#46528DepositorInsertVav1 (VAV1 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 661-777PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-99b-5p
Plasmid#103767PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-99b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-99b-5p target (MIR99B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125b-5p
Plasmid#103191PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125b-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nck2-SH2
Plasmid#46458DepositorInsertNM_001004720.2 (NCK2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 274-380PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-302c-3p
Plasmid#103403PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-302c-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-302c-3p target (MIR302C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_YFP
Plasmid#45278DepositorInsertYFP
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4-K129R (GST)
Plasmid#21564DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
UseTagsGSTExpressionMammalianMutationLysine 129 to argininePromoterAvailable sinceJuly 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX Eat2-SH2
Plasmid#46423DepositorInsertEat2 (SH2D1B Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-132PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only