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Plasmid#51892PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorInsertcry1 (Cry1 Mouse)
UseTags3x Flag and mycExpressionMutationPromoterpolyhedrin promoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)
Plasmid#110146PurposeTransformation and expression of Csy4 and cogDsRed proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogRFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX chimerin2-SH2
Plasmid#46416DepositorInsertchimerin2 (CHN2 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 49-155PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Mist-SH2 RK
Plasmid#46455DepositorInsertMist RK (Clnk Mouse)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 300-435PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep70
Plasmid#136870PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep70 (CEP70 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ASAP1-1/1
Plasmid#91501PurposeProtein expression and purification of human SH3 domain construct ASAP1-1/1DepositorInsertASAP1-1/1 (ASAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep70
Plasmid#136871PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep70 (CEP70 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep152
Plasmid#136874PurposeMammalian expression of the centrosomal protein Cep152 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep152 (CEP152 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2-SH2 RK
Plasmid#46441DepositorInsertGrb2 RK (GRB2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 58-159PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-AGO1 ts1
Plasmid#115850PurposeAGO1 knockdownDepositorInsertAGO1 shRNA (AGO1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep41
Plasmid#136843PurposeMammalian expression of the centrosomal protein Cep41 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep41 (CEP41 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEa
Plasmid#121900PurposePlasmid for making zebrafish apoEa in situ probeDepositorInsertApoEa (apoea Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep97
Plasmid#136850PurposeMammalian expression of the centrosomal protein Cep97 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep97 (CEP97 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Cep97
Plasmid#136801PurposeMammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep97 (CEP97 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A-Q482A
Plasmid#128946Purposeexpresses R480A Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOCK5-1/1-IS1/2
Plasmid#91303PurposeProtein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2DepositorInsertDOCK5-1/1-IS1/2 (DOCK5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNMBP-6/6
Plasmid#91489PurposeProtein expression and purification of human SH3 domain construct DNMBP-6/6DepositorInsertDNMBP-6/6 (DNMBP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_KALRN-1/4
Plasmid#91283PurposeProtein expression and purification of human SH3 domain construct KALRN-1/4DepositorInsertKALRN-1/4 (KALRN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X* (AcrIIC1X-mCherry chimera)
Plasmid#128114PurposeExpresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9DepositorInsertAcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
Plasmid#110145PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only