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Plasmid#91052PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSPDepositorInsertCsy4-P2A-dAtCas9-VPR
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191182PurposeExpression of EACTB sensor in mammalian cellsDepositorInsertmCherry:ACTB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191184PurposeExpression of RPLP1 sensor in mammalian cellsDepositorInsertmCherry:RPLP1.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)
Plasmid#191175PurposeMammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).DepositorInsertCMV-TO:mCherry:s70587:EGFP (frameshifted)
UseTagsExpressionMammalianMutationEGFP is frameshiftedPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx C35S
Plasmid#36947DepositorInsertThioredoxin-1 C35S mutation (TXN Human)
UseTagsHis tagExpressionBacterialMutationcys 35 to serinePromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL2
Plasmid#67053PurposeStandard for cell-free expression benchmarking.DepositorInsertIL2 (IL2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBDK
Plasmid#135190PurposeIntegration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selectionDepositorInsertKanR
UseSynthetic BiologyTagsExpressionBacterialMutationPromotercatAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB045 (ZapB-mEos2)
Plasmid#72651PurposeInducible expression of ZapB-mEos2 in bacteriaDepositorInsertZapB-mEos2
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag Snail 4SA
Plasmid#16220DepositorInsertSnail (SNAI1 Human)
UseTagsFlagExpressionMammalianMutation4SA: S108A, S112A, S116A, S120APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAID-CMV linearizing in pX330
Plasmid#140609PurposeCrispr/Cas9 plasmid for linearization of pAID-CMVDepositorInsertsgRNA for pAID-EF1a plasmid digestion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB6677
Plasmid#106155PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-Trpa1b-2A-mCherry
Plasmid#106291PurposeGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherryDepositorInsertTrpa1b (trpa1b Zebrafish)
UseGateway middle entryTags2A-mCherryExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Renilla
Plasmid#79496PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDSDepositorInsertRenilla Luciferase
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDM1516
Plasmid#109001Purposesafe haven knock-in plasmid for the act5 locus, C-terminal mCherry taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, c…TagsmCherryExpressionMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAT.011
Plasmid#119555PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Firefly
Plasmid#79495PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDSDepositorInsertFirefly Luciferase
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW414hrpL-cIgfp
Plasmid#61439PurposepSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs34-CI-LVA-t-plam-30gfp-t
UseTagsLVAExpressionBacterialMutationPromoterHrpLAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Gata1
Plasmid#41085DepositorInsertGata1 (Gata1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetO + minimal CMVAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A
Plasmid#171785PurposeExpression of HaloTag N58-(GGS)X6-hH2A in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-hH2A
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9_1
Plasmid#91156PurposeCas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only