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Plasmid#89155PurposeRecombinant expression of PTBP1-RRM12 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
UseTagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb10-SH2
Plasmid#46437DepositorInsertGrb10 (GRB10 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 435-548PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-Xyl-Xmod-DocIII
Plasmid#60865PurposeCharacteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120CDepositorInsertsXyl
Xmod-DocIII
UseTags6xHISExpressionBacterialMutationThreonine 120 to Cysteine in Xylanase domainPromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LASP1-1/1-SV1/2
Plasmid#91399PurposeProtein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2DepositorInsertLASP1-1/1-SV1/2 (LASP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(C)-SH2
Plasmid#46431DepositorInsertGap/RASGAP(C) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 339-452PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-GGGS
Plasmid#162610PurposeAllows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
UseTagsExpressionMammalianMutationContains aa1-155 of mVenusPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-N-Flag-hCRACR2A-isoform-a-Q604L
Plasmid#79595PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationQ604LPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
MO70-N-Flag-hCRACR2A-isoform-a-T559N
Plasmid#79594PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationT559NPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRAF_S29A_pcDNA3.1
Plasmid#86506PurposeExpresses CRAF_S29A in mammalian cells [Edit]DepositorInsertCRAF (RAF1 Human)
UseTagsFLAGExpressionMammalianMutationS29APromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-2/2-SV2/2
Plasmid#91343PurposeProtein expression and purification of human SH3 domain construct CRK-2/2-SV2/2DepositorInsertCRK-2/2-SV2/2 (CRK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(K271Q)
Plasmid#133273PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsHAExpressionMammalian and YeastMutationK271QPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167848PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
UseTags6xHis and AviTagExpressionBacterialMutationPromoterT7Available sinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX Nsp2-SH2
Plasmid#46460DepositorInsertNsp2 (BCAR3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 144-259PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-1/2
Plasmid#91480PurposeProtein expression and purification of human SH3 domain construct GRB2-1/2DepositorInsertGRB2-1/2 (GRB2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-2/2
Plasmid#91410PurposeProtein expression and purification of human SH3 domain construct GRB2-2/2DepositorInsertGRB2-2/2 (GRB2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2-2/2
Plasmid#91368PurposeProtein expression and purification of human SH3 domain construct GRAP2-2/2DepositorInsertGRAP2-2/2 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgGFP_3
Plasmid#78165PurposesgGFPDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EPS8L2-1/1
Plasmid#91526PurposeProtein expression and purification of human SH3 domain construct EPS8L2-1/1DepositorInsertEPS8L2-1/1 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
KAT6B-Tol2
Plasmid#113384Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B geneDepositorInsertKAT6B-enhancer (KAT6B Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only