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Plasmid#58776PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0908
Plasmid#91027PurposeModule A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSPDepositorInsertTREX2-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pconst #1-RFP
Plasmid#81075Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23119Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDM1514
Plasmid#108999Purposesafe haven knock-in plasmid for the act5 locus, N-terminal mCherry taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, n…TagsmCherryExpressionMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-CmCherry-mAID
Plasmid#140604PurposeExpression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP
Plasmid#199456PurposeExpression of stdPCP-PH in mammalian cellsDepositorInsertstdPCP-PH
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
UseTagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14_GFP5_Encap
Plasmid#86408PurposeExpresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 5aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x4
Plasmid#58774PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1-Ni
Plasmid#17744DepositorInsertPIK3R1-Ni (PIK3R1 Human)
UseTagsExpressionInsectMutationPartial cDNAPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-2X snRP1
Plasmid#99688PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-2x
Plasmid#172282PurposeExpressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequenceDepositorInsertEGFP-N1-2x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-4x
Plasmid#172283PurposeExpressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequenceDepositorInsertEGFP-N1-4x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-CmYLCV-6
Plasmid#197699PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCmYLCV promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-RLuc-9
Plasmid#197739PurposePhytobrick (MoClo) Level 0 PartDepositorInsertRenilla luciferase (tomato optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)
Plasmid#191151PurposeExpression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L72:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIC-lyn11-myc-LOV2GIVe (FA)
Plasmid#170626PurposeFor the mammalian expression of mLOV2GIVe GEF-deficient mutation F1685ADepositorInsertLyn11-myc-LOV2GIVe (FA)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
VV201: ChR2(C128S)-eGFP in fubi
Plasmid#58510PurposeExpresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoterDepositorInsertChR2(C128S)-eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Cysteine 128 to SerinePromoterUbiquitinAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only