-
Plasmid#125574PurposeExpresses Cre under the CAG promoter in mammalian cells.DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
KA1158_pPBCAG-MerCreMer-IZ
Plasmid#124184PurposepiggyBac-based expression of the tamoxifen-inducible CreDepositorInsertMerCreMer-IRES-Zeo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
linker-mCitrine-PIF6-NAT
Plasmid#51576PurposeTag protein of interest with PIF6 in Saccharomyces cerevisiaeDepositorInsertPIF6 (PIL2 Mustard Weed)
UseTagslinker and mCitrineExpressionYeastMutation1-100aa of PIF6PromoternoneAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_INT
Plasmid#68436PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc_SV40:PuroR
Plasmid#183038PurposeExpresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-75-2
Plasmid#73036PurposeExpress an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cellsDepositorInsertp75-2
UseLentiviralTagsExpressionMammalianMutationMature Human NT-3 gene exchanged Aspartic 15 to a…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TPC2-D276K-G-GECO1.2
Plasmid#207142PurposeFusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationChanged Aspartate 276 to LysinePromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F0
Plasmid#184162PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1DA
Plasmid#35028DepositorInsertFEN1DA (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationD181A--NUCLEASE DEAD POINT MUTANTPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS ColIII alpha1
Plasmid#19187DepositorInsertProcollagen type III alpha 1 (COL3A1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-EV2.1
Plasmid#158411PurposeCRISPR-dpcoCas9 fused with EDLL activator for transcriptional activationDepositorInsertdpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor_eGP2AP_RC
Plasmid#133784PurposeLibrary reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.DepositorTypeEmpty backboneUseTagseGFP-P2A-PuroExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MKRN1_H307E
Plasmid#78756PurposeTo overexpress MKRN1_H307E in Mammalian CellDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutationH307EPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CDC10
Plasmid#51568PurposeConstitutively express bud neck localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCDC10 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNLuc_SV40:PuroR
Plasmid#183036PurposeExpresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA1875_pGL4-UBCmin-luc2/Sox2_SRR2
Plasmid#124198PurposeLuciferase reporter driven by the Sox2 enhancerDepositorInsertSox2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only