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Plasmid#63063PurposeMammalian expression of EGFP-TEV-S-tag hNSL1 for protein localization and affinity purification (LAP)DepositorInserthNSL1, IMAGE clone 3997280 (NSL1 Human)
UseTagsEYFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIC65
Plasmid#63061PurposeRetroviral expression of EGFP-TEV-S-tag Mis12 for protein localization and affinity purification (LAP)DepositorInserthMis12, IMAGE clone 3351245 (MIS12 Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationL68RPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EYFP-ccdB-EBFP2
Plasmid#158553PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 SS-AA
Plasmid#13697DepositorInsertHPV 16 E7 S31A, S32A (E7 Human)
UseTagsExpressionMammalianMutationaa 31 S to A, aa 32 S to APromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 SS-DD
Plasmid#13704DepositorInsertHPV 16 E7 S31D, S32D (E7 Human)
UseTagsExpressionMammalianMutationaa 31 S to D, aa 32 S to DPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-
Plasmid#191390PurposeDonor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locusDepositorInserthCdt1(1-100)Cy-
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-EYFP
Plasmid#158558PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-1BPNLS-Cas9-1BPNLS
Plasmid#87108PurposeBPNLS-Cas9-BPNLS expression plasmidDepositorInsert1BPNLS-Cas9-1BPNLS
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_LAP3_WT_V5
Plasmid#82958PurposeGateway Donor vector containing LAP3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertLAP3 (LAP3 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_BSD_FRT-PuroR
Plasmid#177867PurposeCloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassetteDepositorInsertBSD-based EXSISERS
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsOLLASExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CBR3_WT_V5
Plasmid#83015PurposeGateway Donor vector containing CBR3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertCBR3 (CBR3 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRep-Cap Scanning Saturation Mutagenesis (SSM) pooled library
Pooled Library#199601PurposepRep-Cap SSM library enables VP1, VP2, and VP3 expression under the control of the endogenous p40 promoterDepositorExpressionMammalianSpeciesAav2UseAAVAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human Whole Genome sgRNA iBAR Library
Pooled Library#140633PurposeCRISPR pooled screen at high MOI by the embedded internal barcodesDepositorUseCRISPR and LentiviralAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mode #2 Library (NEDD4-2 WW2 domain)
Pooled Library#111708PurposeEncodes NEDD4-2 WW2 domain and human phosphopeptides fused to split mCherry for Hi-PDepositorExpressionBacterialSpeciesHomo sapiensAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-dTom-nlsdTom (AAV9)
Viral Prep#135630-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-ChR2-mCherry (AAV PHP.eB)
Viral Prep#135634-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-Gq-P2A-dTomato (AAV9)
Viral Prep#135635-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-Gq-P2A-dTomato (#135635). In addition to the viral particles, you will also receive purified pAAV-S5E2-Gq-P2A-dTomato plasmid DNA. Expression of Gq-DREADD and dTomato (physically separate) under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomato (physically separate, not a fusion protein)Available sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GFP-fGFP (AAV1)
Viral Prep#135631-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsEGFPAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-dTom-nlsdTom (AAV PHP.eB)
Viral Prep#135630-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GFP-fGFP (AAV9)
Viral Prep#135631-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsEGFPAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only