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Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
CKAP2-mNeonGreen pHAT
Plasmid#175947PurposeBacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorInsertCKAP2 (Ckap2 Mouse)
UseTags6His, StrepTagII, and mNeonGreenExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX17WHC
Plasmid#118839PurposeRetroviral expression of a mutant of Sox17 that improves efficiency of iPSC generationDepositorInserteSOX17WHC (Sox17 Mouse)
UseRetroviralTagsExpressionMammalianMutationWHC: L46W, V53H, E57CPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX17FNV
Plasmid#118838PurposeRetroviral expression of a mutant of Sox17 that improves efficiency of iPSC generationDepositorInserteSOX17FNV (Sox17 Mouse)
UseRetroviralTagsExpressionMammalianMutationFNV: L46F, V53N, E57VPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sema5a-AP-His
Plasmid#72035PurposeExpresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema5a (Sema5a Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
E1010m (RFP)_CD
Plasmid#66033PurposeMoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]DepositorInsertFluorescent reporter - RFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR/U6 HDAC4 shRNA
Plasmid#32220DepositorInsertshRNA against murine HDAC4 (Hdac4 Mouse)
UseRNAi; Gateway u6 entry vectorTagsExpressionMutationPromoterhuman U6Available sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Sema4b-Fc-His
Plasmid#72155PurposeExpresses the extracellular region of the Sema4B protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema4b (Sema4b Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp1-AP-His
Plasmid#71971PurposeExpresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNrp1 (Nrp1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K227/230R
Plasmid#127135DepositorInsertRPS3 (RPS3 Human)
UseLentiviralTagsExpressionMutationK227R/K230RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K230R
Plasmid#127134DepositorInsertRPS3 (RPS3 Human)
UseLentiviralTagsExpressionMutationK230RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K227R
Plasmid#127133DepositorInsertRPS3 (RPS3 Human)
UseLentiviralTagsExpressionMutationK227RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVRa12_807
Plasmid#49663DepositorInsertECF12_807
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_2
Plasmid#103920PurposeProtein expression and purification of PTN13 PDZ2 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_5
Plasmid#103923PurposeProtein expression and purification of PTN13 PDZ5 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE123 XDsh delta PDZ-GFP- CS2+
Plasmid#16786DepositorInsertXdsh-delta-PDZ (dvl2.L Frog)
UseXenopus expressionTagsgfpExpressionMutationcontains Dsh amino acids 1-267 and 381-736 (delet…PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only
