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Plasmid#38266DepositorInsertautophagy related genes 14 (ATG14 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged A618T, A621T, C624A and T627C for siRNA r…PromoterLTRAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (830-839)
Plasmid#188091PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationATG9 (830-839)PromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep41
Plasmid#136794PurposeMammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep41 (CEP41 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG
Plasmid#74263PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.R404X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-α-tubulin
Plasmid#136791PurposeMammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tagDepositorInsertSNAP-TUBA (DNMBP Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook3
Plasmid#198525PurposeExpression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook3 (HOOK3 Human)
UseTagsGAL4-DNA binding domain (BD) fragmentExpressionYeastMutationPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-γ-tubulin
Plasmid#136799PurposeMammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tagDepositorInsertSNAP-TUBG (TUBG1 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEb
Plasmid#121901PurposePlasmid for making zebrafish apoEb in situ probeDepositorInsertApoeb (apoeb Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD111
Plasmid#78166PurposeExpresses Cas9 with the pLX311 backboneDepositorInsertCas9
UseLentiviralTags3xFLAG and NLSExpressionMutationPromoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pdpy-30-miniCAFE
Plasmid#170115PurposeDpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.DepositorInsertMiniCAFE
UseCRISPRTagsExpressionMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterDpy-30Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-His
Plasmid#67866Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tagDepositorInsertMRPP3 (KIAA0391 Human)
UseTagsHisExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nsp14D-mCherry
Plasmid#165139Purposemammalian expression and localizationDepositorInsertNsp14D (ORF1ab codon optimized SARS-COV-2)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp4 deltaC-EGFP
Plasmid#165126Purposemammalian expression and localizationDepositorInsertNsp4 deltaC (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167849PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
3xNLS-NLP-cMyc-cMyc enAspCas12a
Plasmid#182127PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc enAspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-C (TRP1)
Plasmid#177795PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV-miR-142
Plasmid#117056PurposeOverexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expressionDepositorInsertmiR-142-3p - OFP (Mir142 Mouse)
UseLentiviralTagsExpressionMutationPromoterSFFV promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep70
Plasmid#136821PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep70 (CEP70 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2
Plasmid#176252PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by fullDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
[UBC][EIF4e][GG cloning]
Plasmid#97189PurposeEIF4e plasmid with Pum cloning siteDepositorInsertEIF4E variant 1 (EIF4E Human)
UseSynthetic BiologyTagsempty Pum cloning siteExpressionMammalianMutationPromoterUBC (ubiquitin C)Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only