Addgene

We narrowed to 28,098 results for: eng

Showing: 11201 - 11220 of 28098 results

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pMXs-PT2A-iRFP670-GFP-Tdtomato

Plasmid

#111816

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
GFP
Tdtomato

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65(100-261)-RTA(125-190)

Plasmid

#99673

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

VP64-p65(101-261)-RTA(125-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pWXLd-RIEP-OgNLuc

Plasmid

#135936

Purpose

Expresses the OgNLuc LumiFluor in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA-GFP-R5

Plasmid

#106256

Purpose

Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide

Depositor

Urartu Seker

Article

Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

Insert

Green fluorescent protein

Use

Tags

Added silaffin R5 peptide to C terminus of GFP af…

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pML3009

Plasmid

#193256

Purpose

Gamma-aminobutyric acid sensor expressing eYFP reporter

Depositor

Lauren Andrews

Article

Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

Inserts

gabR
eYFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

synthetic promoter P2 and synthetic promoter PGab…

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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p426 25Q GPD

Plasmid

#1181

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pFLAG-ING2

Plasmid

#13295

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)

Plasmid

#191144

Purpose

Expression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ136-tRNA2.0

Plasmid

#158398

Purpose

Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2 3xmiR-122T

Plasmid

#35672

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

Luciferase

Tags

Expression

Mutation

Promoter

HSV-TK

Available since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1A

Plasmid

#183337

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL-neo delta-p85

Plasmid

#10888

Depositor

William Hahn

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

delta-p85 (PIK3R1 Bovine)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Deletion of aa 478-513

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_VEGFB

Plasmid

#183328

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFA

Plasmid

#183327

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRB

Plasmid

#183314

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRB

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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p426 103Q GAL

Plasmid

#1188

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pMG

Plasmid

#58991

Purpose

MSCV based expression vector - expresses GFP

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Type

Empty backbone

Use

Retroviral; Mscv-based retroviral system that exp…

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
Lyn-FRB-GFP11

Plasmid

#181863

Purpose

Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

Lyn-FRB-GFP11

Use

Tags

GFP11 and N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ING2

Plasmid

#13294

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only