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Plasmid#171654PurposeQUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-10-T7
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-15-T7-GFP_ColE1
Plasmid#171655PurposeQUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertQUAS-15-T7
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-5-QUAS-GFP_ColE1
Plasmid#171656PurposeQUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.DepositorInsertT7-5-QUAS
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-mNG
Plasmid#189722Purposetitratable expression of mNeonGreen in bacteriaDepositorInsertmNeonGreen
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIR23)-sfGFP
Plasmid#189676Purposetitratable expression of sfGFP in bacteriaDepositorInsertsfGFP
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-pvdF WT
Plasmid#187974PurposeExpresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosaDepositorInsertpvdF WT
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODALvar
Plasmid#115640PurposeFor targeted integration and inducible expression of a human NODAL splice variant using doxycyclineDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matMYC-C312S
Plasmid#115256PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal MYC tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationC312SPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matMYC-ex2trunc
Plasmid#115257PurposeFor mammalian expression of a C-terminal truncated human NODAL open reading frame with an internal MYC tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationC-terminal truncationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matDYK-C312S
Plasmid#115259PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationC312SPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matDYK
Plasmid#115260PurposeFor mammalian expression of a human NODAL splice varaint open reading frame with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-T7TS-C312S
Plasmid#115263PurposeFor in vitro transcription of human NODAL open reading frame (with a mutated Cysteine residue) RNADepositorInsertNODAL (NODAL Human)
UseTagsExpressionBacterialMutationC312SPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-matNODALvar-HIS
Plasmid#115265PurposeFor bacterial recombinant protein production of the mature peptide of a human NODAL splice variantDepositorInsertNODAL variant mature peptide (NODAL Human)
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK
Plasmid#115245PurposeFor mammalian expression of a human NODAL splice variant open reading frame with a C-terminal MYC-DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matMYC
Plasmid#115249PurposeFor mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tagDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-T7TS
Plasmid#115251PurposeFor in vitro transcription of a human NODAL splice variant open reading frame RNADepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
His8OphMA-21mer.
Plasmid#189670PurposeHis8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.DepositorInsertHis8OphMATEV18mer
UseTagsHis8 taggedExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
His8OphMADeltaC6-18mer.
Plasmid#189671PurposeHis8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptidesDepositorInsertHis8OphMA∆C6-TEV18mer
UseTagsHis8 taggedExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF4E9D
Plasmid#185844PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>LacI>TUP1>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9C3
Plasmid#185843PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>RevTetR_r1.7>SIN3C>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only