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Plasmid#61206PurposeFluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPDLP1
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
TS module
Plasmid#26021DepositorInsertTension Sensor module
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMB-PDESr
Plasmid#61207PurposeFluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPDLP1
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
SMA-Cherry
Plasmid#110209PurposeMapping of mesenchymal progenitor cells.DepositorInsertmCherry
UseTagsNoneExpressionMammalianMutationPromoterSmooth muscle alpha-actin and first intronAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAZ10::LUC
Plasmid#141172PurposeLuminescent reporter for jasmonic acid signalingDepositorInsertAtJAZ10 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtJAZ10, AT5G13220Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Actin Binding Domain Tensionless Control
Plasmid#215371PurposeExpresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).DepositorInsertActin Binding Domain Tensionless Control
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AKARdual
Plasmid#99869PurposeMeasure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertAKAR4
UseTagsLSSmOrange and mKate2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc
Plasmid#208851PurposeExpresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsV5 Epitope TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s
Plasmid#100020PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.DepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34W)-PB
Plasmid#171072PurposeA COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY34WPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-acVHH (Y34F)-PB
Plasmid#171071PurposeA COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY34FPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH (Y104F)–PB
Plasmid#169683PurposeA COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY104FPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138I, T78HPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I
Plasmid#100922PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only