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Showing: 1101 - 1120 of 2436 results

  1. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  2. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  3. C105m

    Plasmid
    #121008
    Purpose
    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-AAV ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    AAV degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121008-plasmid-map-sequence-id-236163

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  4. C106m

    Plasmid
    #121009
    Purpose
    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-DAS ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    DAS degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121009-plasmid-map-sequence-id-236172

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  5. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

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  6. C70m

    Plasmid
    #120982
    Purpose
    MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).
    Depositor
    Richard Murray
    Insert
    RpaR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120982-plasmid-map-sequence-id-236041

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  7. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  8. C61m

    Plasmid
    #120977
    Purpose
    MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaR from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120977-plasmid-map-sequence-id-236031

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  9. C62m

    Plasmid
    #120978
    Purpose
    MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TraR(W) from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120978-plasmid-map-sequence-id-236032

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  10. P61m

    Plasmid
    #120953
    Purpose
    MoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).
    Depositor
    Richard Murray
    Insert
    Tac promoter Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120953-plasmid-map-sequence-id-235967

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  11. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120940-plasmid-map-sequence-id-238036

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  12. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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  13. P35m

    Plasmid
    #120946
    Purpose
    MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).
    Depositor
    Richard Murray
    Insert
    pLbda-CasRQi
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120946-plasmid-map-sequence-id-235948

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  14. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

    Plasmid
    #195048
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195048-plasmid-map-sequence-id-386137

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  15. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

    Plasmid
    #195047
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195047-plasmid-map-sequence-id-386131

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  16. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

    Plasmid
    #195046
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195046-plasmid-map-sequence-id-386136

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  17. pHEE-R-bar9si

    Plasmid
    #222834
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222834-plasmid-map-sequence-id-446485

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  18. pBHt2G-RFP

    Plasmid
    #107162
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Type
    Empty backbone
    Use
    Fungal grobacterium transformation plasmid, teste…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    107162-plasmid-map-sequence-id-205976

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  19. C67m

    Plasmid
    #120981
    Purpose
    MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bxb1 integrase - mScarlet-i - LAA tag
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120981-plasmid-map-sequence-id-236036

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  20. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

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Showing: 1101 - 1120 of 2436 results