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Addgene
Showing: 1101 - 1120 of 1239 results
  1. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid
    #119189
    Purpose
    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
    Depositor
    Insert
    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Availability
    Academic Institutions and Nonprofits only
  2. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Availability
    Academic Institutions and Nonprofits only
  3. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.
    Availability
    Academic Institutions and Nonprofits only
  4. pJMH058

    Plasmid
    #186640
    Purpose
    aadA.co-TaadA-PcpcB-eYFP.co between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-PcpcB-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pJMH059

    Plasmid
    #186641
    Purpose
    aadA.co-TaadA-PrbcL-eYFP.co between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-PrbcL-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pJMH139

    Plasmid
    #186642
    Purpose
    aadA.co-TaadA-PJ23119-eYFP.co between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-PJ23119-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pJMH140

    Plasmid
    #186643
    Purpose
    aadA.co-TaadA-PJ23111-eYFP.co between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-PJ23111-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pJMH141

    Plasmid
    #186644
    Purpose
    aadA.co-TaadA-PJ23100-eYFP.co between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-PJ23100-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pJMH142

    Plasmid
    #186645
    Purpose
    aadA.co-TaadA-PJ23107-eYFP.co between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-PJ23107-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pJMH143

    Plasmid
    #186646
    Purpose
    aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA
    Depositor
    Insert
    aadA.co-TaadA-PJ23117-eYFP.co
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Availability
    Academic Institutions and Nonprofits only
  12. pAAV-CAG-DIO-PPO-Venus

    Plasmid
    #139504
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Has Service
    AAV5
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
Showing: 1101 - 1120 of 1239 results