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Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
PPRE X3-TK-luc
Plasmid#1015PurposePPAR reporter. 3xDR1 sites upstream of a luciferase reporter.DepositorInsertPPRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Basic Vector
Plasmid#212936PurposeFirefly luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CROPseq-puro-v2
Plasmid#127458PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT
Plasmid#207832Purposetelomere specific nuclease to create double-strand breaks at telomeresDepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFlag, DD, ER, mCherry and FokIExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-I3_C1
Plasmid#189756PurposeC1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent proteinDepositorInsertmScarlet-I3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A
Plasmid#207953Purposenuclease inactive control for TRF1FokI WTDepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFlag, DD, ER, mCherry and FokI (D450A)ExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
gag-MCP-pol
Plasmid#211370PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-MCP-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/1
Plasmid#112862PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap1
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ:RUBY
Plasmid#160909PurposeRUBY under the control of Ubiquitin promoter/marker for transformationDepositorInsertRUBY and UBIQUITIN promoter
UseTagsExpressionPlantMutationPromoterUBIQUITINAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V
Plasmid#63586PurposepsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.DepositorInsertPackaging plasmid for integrase deficient lentiviral vectors
UseIntegrase deficient lentiviral packaging vectorTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-cOVA-IRES-BFP
Plasmid#135074PurposeExpresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.DepositorInsertcOVA-IRES-BFP (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-Opti
Plasmid#163126PurposeLentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8s (AAV9)
Viral Prep#169256-AAV9PurposeReady-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8s (#169256). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8s plasmid DNA. hSyn-driven expression of soma-targeted calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR2-FusionRed (AAV1)
Viral Prep#105669-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsFusionRedAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lact-C2-GFP
Plasmid#22852DepositorInsertLactadherin-C2 Domain (MFGE8 Bovine)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2010AvailabilityAcademic Institutions and Nonprofits only