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pcDNA3.1-GAVPO
Plasmid#159923PurposeGAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133DepositorInsertGAVPO
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAX-SCLM
Plasmid#216757PurposeFor strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoterDepositorInsertSuperClomeleon
UseTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCAG (cytomegalovirus-chicken beta-actin-rabbit be…Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cyto-V5-APEX2
Plasmid#170572PurposeExpresses APEX2 in cytosolDepositorInsertAPEX2
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP3A4
Plasmid#67104PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP3A4 (CYP3A4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATGL*-V5-APEX2
Plasmid#170574PurposeA C-terminal APEX2 fusion to the human ATGL S47A proteinDepositorInsertATGL* (PNPLA2 Human)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationchanged serine 47 to alaninePromoterCMVAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
TVT7R(0,0)
Plasmid#98631PurposeVector for cloning infectious RNA virus genomesDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE311B
Plasmid#120418PurposeExpresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanRDepositorArticleInsertsMutL E32K
Lambda Beta
XylS
UseTagsExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterPmAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEvol-NnSULT1C1-cysDNCQ
Plasmid#188983Purposebiosynthesize sulfotyrosine in E. coli.DepositorInsertsNnSULT1C1
cysD
cysN
cysC
cysQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GtACR2-ChRmine-tandem-eYFP
Plasmid#192580PurposeAAV-vector for bidirectional optogenetic manipulation of neuronsDepositorInsertsomBiPOLES
UseAAVTagsEYFP- soma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSV Sport SREBP-1c
Plasmid#8883DepositorInsertSREBP-1c (Srebf1 Mouse)
UseTagsExpressionMammalianMutationActive version of SREBP-1c.PromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
APEX2-V5-G3BP1_pTRE-G418
Plasmid#202013Purposeexpresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertAPEX2-V5-G3BP1
UseLentiviralTagsExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB80 (R26-CreER)
Plasmid#12168DepositorInsertRosa26 CreER^T2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ACPs (from S. pneumonia)
Plasmid#63687PurposeInducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniaeDepositorInsertacpS
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ma-PylRS-lib1
Pooled Library#224585PurposePyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins.DepositorExpressionBacterialSpeciesMethanomethylophilus alvusAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAF0035 Ef1a-Nm60-2a-EGFP
Plasmid#193464PurposeExpression of RecombinaseDepositorInsertNm60
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV JMJD3 mutant
Plasmid#21214DepositorInsertJMJD3 (KDM6B Human)
UseRetroviralTagsFlag and HAExpressionMammalianMutationH1390APromoterAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF1307
Plasmid#142371PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertJARID2 (JARID2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)
Plasmid#190112PurposeAAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFPDepositorInsertsMMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
UseAAVTagsbpNLS and bpNLS-P2A-eGFPExpressionMutationmutations from RT in PE2 and truncation of RNAse …PromoterEFS and U6, H1Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOE-E3-T2A-mCherry
Plasmid#190808PurposeExpress APOE E3 allele from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE3PromoterUbi CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only
