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Plasmid#166085PurposePlasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertLYS9 (LYS9 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pR18/pL18-ccdB
Plasmid#164899PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-TFSumo
Plasmid#112576PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TFSumo and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-Tsf
Plasmid#112578PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-Tsf and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-AFV
Plasmid#112560PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-AFV and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
GGDest2-Amp
Plasmid#157650PurposeGolden Gate assembly of a pET28-compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
GGDest3-Amp
Plasmid#157651PurposeGolden Gate assembly of a pTXB1 compatible fusion-protein ORF insertDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGEX4-4T-1 BCP1 NTase
Plasmid#161812PurposeExpresses SMODS/CD-NTase from Bacillus phage Bcp1DepositorInsertphage BCP1 SMODS/CD-NTase enzyme
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL1271
Plasmid#129282PurposeExpression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)DepositorInsertalpha-Xylp_1211 (Ga0256695_ 1211)
UseTagsExpressionBacterialMutationPromoterClostridium thermocellum DSM1313 Cellobiose Phosp…Available sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX061
Plasmid#126279PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf04664g02003.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX031
Plasmid#126249PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf00073g05010.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX006
Plasmid#126224PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf23241g00003.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX083
Plasmid#126301PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf05324g01002.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX176
Plasmid#126394PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf00683g00003.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX180
Plasmid#126398PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf08298g03010.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX147
Plasmid#126365PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf06228g01001.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only