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Plasmid#42554DepositorInsertshBIRC5 (BIRC5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nsp13 K289A-EGFP
Plasmid#165130Purposemammalian expression and localizationDepositorInsertNsp13 K289A (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp15
Plasmid#165120Purposemammalian expression and localizationDepositorInsertNsp15 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(N)-SH2
Plasmid#46432DepositorInsertGap/RASGAP(N) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 168-283PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(N)-SH2
Plasmid#46498DepositorInsertSHP-2(N) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-104PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
shBIRC5 # 1
Plasmid#42553DepositorInsertshBIRC5 (BIRC5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-Jun4A-Myc
Plasmid#47444Purposemammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)DepositorInsertJun 4A mutant (Jun Mouse)
UseTagsFlag and mycExpressionMammalianMutationfour N-terminal residues phosphorylated by JNK, s…PromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry+NLS
Plasmid#128013PurposeVector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4 DBD-SV40NLS-3x-Flag
UseGal4 entry vector with nls to generate tethering …TagsGal4-DBD-SV40NLS-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-K508M
Plasmid#191753PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationc.1523A>T (p.K508M) + silent mutation c.1515A&…PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-ybbR
Plasmid#60866PurposeCharacteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tagDepositorInsertsCohIII
CBM
UseTags6xHIS, HRV 3C, and ybbRExpressionBacterialMutationCysteine 63 to Serine in CBM modulePromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
UCP3 promoter EP1
Plasmid#71743PurposeContains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activityDepositorInsertUncoupling Protein 3 promoter (Ucp3 Mouse)
UseTagsLuciferaseExpressionBacterialMutationContains 7kb of the murine UCP3 gene encompassing…PromoterUCP3Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorInsertHSPA5 promoter (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot8-D40AE42A_AH
Plasmid#148902PurposeMammalian Expression of HsNot8-D40AE42ADepositorInsertHsNot8-D40AE42A (CNOT8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorInsertHis6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13_GC3opt
Plasmid#157715Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTet-SNAP-Cep78
Plasmid#136827PurposeMammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep78 (CEP78 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG
Plasmid#74261PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.Y591X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-ALMS1
Plasmid#136828PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tagDepositorInsertSNAP-ALMS1 (ALMS1 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only