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Plasmid#30225DepositorInsertrhVitronectin with N terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 20*-62PromoterAvailable sinceMay 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Sa-SlugCas9
Plasmid#163797PurposeExpresses Sa-SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)
Plasmid#159853PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(PATC)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF2
Plasmid#200379PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-WP1-CRY2PHR-VP64
Plasmid#171758PurposeLentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64DepositorInsertWP1-CRY2PHR-VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-iRFP670-GFP
Plasmid#111815PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
iRFP670
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV- CAG-h2BmRuby2FLAG
Plasmid#116872PurposeNuclear localized mRuby2FLAGDepositorInsertH2BmRuby2FLAG
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTFP1-ShadowG
Plasmid#99867PurposeMeasure FRET between mTFP1 and ShadowGDepositorInsertShadowG
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT276
Plasmid#122607Purposeexpress CDA1-BE4max in mammalian cellsDepositorInsertCDA1-BE4max
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1afull-H2B-mCherry-P2A-Fluc
Plasmid#207253PurposeLentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoterDepositorInsertH2B-mCherry-P2A-Fluc
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alpha_fullAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FLT3
Plasmid#183293PurposeAll-in-One CRISPRko system with a guide RNA that targets FLT3 geneDepositorInsertFLT3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFC331
Plasmid#87843PurposeAMA1 plasmid with Aspergillus optimized Cas9 and argB selection markerDepositorInsertsCas9
argB
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP
Plasmid#67299PurposeBlue light dependent photoswitching systemDepositorInsertTiam1-pMagFast2(3x)-iRFP (Tiam1 Synthetic, Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
LRP6 deltaN-pCS2-VSVG
Plasmid#27281DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionTagsVSVGExpressionMutationcontains amino acid residues 1370–1613 (extracell…PromoterAvailable sinceJan. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGRCRE
Plasmid#129116PurposeExpresses cre recombinase in Z. rouxii yeast cellsDepositorInsertcre
UseCre/LoxTagsExpressionYeastMutationPromoterS. cerevisiae GAL1 promoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-syn pA
Plasmid#99689PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_PDGFRA
Plasmid#183313PurposeAll-in-One CRISPRko system with a guide RNA that targets PDGFRA geneDepositorInsertPDGFRA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)
Plasmid#191179PurposeNegative control sensor in human cells.DepositorInsertmCherry:Bdnf.s2:EGFP (negative control)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only