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We narrowed to 28,098 results for: eng

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BiP 2: C. elegans BiP (hsp-4)-GFP

Plasmid

#21896

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

BiP (hsp-4 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Fusion between the GFP and hsp-4 takes place at A…

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25_IRES-mCherry

Plasmid

#198386

Purpose

RNF25 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa-VPR mini.

Plasmid

#99685

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pOVC1_EcSSPB-nano_X

Plasmid

#131212

Purpose

Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCBI-LexA-CIB1-biLINuS

Plasmid

#192605

Purpose

Plasmid for constitutive expression of LexA-CIB1-biLINuS.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBDC

Plasmid

#135188

Purpose

Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

CmR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

cat

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET-sCS4-2T

Plasmid

#28250

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain Cse4-Scm3-H4 with 2 Thrombin site

Use

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between Cse4 and Scm3. An…

Promoter

Available since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mOtop3_pcDNA3

Plasmid

#114679

Purpose

This construct was used to express mouse Otop3 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EBFP2

Plasmid

#167905

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 tdAPX W41F

Plasmid

#40863

Purpose

Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

tandem dimer pea APX W41F

Use

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pBLADE_ONLY_C

Plasmid

#168050

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Insert

Blue light-inducible AraC dimers in Escherichia coli

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101**

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBT13-splitC

Plasmid

#122596

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT13-splitD

Plasmid

#122597

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A0108

Plasmid

#91001

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-MSI1(FL)

Plasmid

#60358

Purpose

express mouse MSI1 in mammalian cells

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (FL) (Msi1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pHiUGE V5-epitope donor ORF0

Plasmid

#200377

Purpose

Tagging endogenously expressed proteins with V5 epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

V5-epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSimpleII-NLS-NmCas9-HA-NLS(s)

Plasmid

#47867

Purpose

This plasmid encode NmCas9 with two NLS and a HA tag

Depositor

James Thomson, Erik Sontheimer

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pBTK1003 (pSL2)

Plasmid

#190998

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

CP25

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ135-tRNA2.0

Plasmid

#158397

Purpose

Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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