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GIMAP1 (3LXW)
Plasmid#25336PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGIMAP1:E25-R253 (GIMAP1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids E25-R253PromoterAvailable sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
GIMAP2
Plasmid#28166PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGTPase IMAP family member 2 (GIMAP2 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationAIG1 domain onlyPromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
LisDPS
Plasmid#122654PurposeExpression of LisDPS in bacterial cellsDepositorInsertDPS, listeria
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pNGFP-BC
Plasmid#161956PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pCGFP-BC
Plasmid#161959PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneUseTagsGFPuv His8ExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pML433
Plasmid#177190PurposeExpresses recombinant HyPro enzyme in E. coliDepositorInsertHyPro enzyme
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
S96 ELP
Plasmid#68393PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residueDepositorInsertS96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGatewayTagsExpressionMutationPromoteralpha-Acrystallin promoterAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTtol2pACrymCherry
Plasmid#64023PurposeGateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lensDepositorInsertmCherry
UseGatewayTagsExpressionMutationPromoteralpha-Acrystallin promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Ts-His6
Plasmid#224371PurposeExpress EF-Ts protein for his-tagged purificationDepositorInsertEF-Ts (tsf E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-G-His8
Plasmid#224369PurposeExpress EF-G protein for his-tagged purificationDepositorInsertEF-G (fusA E. coli)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Tu-His8
Plasmid#224370PurposeExpress EF-Tu protein for his-tagged purificationDepositorInsertEF-Tu (tufB E. coli)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-ThrRS
Plasmid#224365PurposeExpress ThrRS protein for his-tagged purificationDepositorInsertThrRS (thrS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-CKM
Plasmid#224375PurposeExpress CKM protein for his-tagged purificationDepositorInsertCKM (LOC107051134 Chicken)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-NDK
Plasmid#224376PurposeExpress NDK protein for his-tagged purificationDepositorInsertNDK (ndk E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-Ferritin-Dronpa
Plasmid#101194PurposeExpresses a Ferritin-Dronpa fusion construct in E. coliDepositorInsertFerritin-Dronpa
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
UseTagsExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
PAP-delta10-cHIS
Plasmid#21716DepositorInsertpolyA polymerase (PAP1 Budding Yeast)
UseTagsHISExpressionBacterialMutation30 amino acids off the c terminusPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-Histag-Avidin
Plasmid#214836PurposeThis plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-Histag-Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only
