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Plasmid#80932PurposeExpresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA(MS2)-puro-barcode
Plasmid#89493PurposeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seqDepositorInsertsgRNA (with MS2 loop)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-TIGHT-EGFP-backward donor
Plasmid#22077DepositorInsertTETO-eGFP
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSub-MBP-GFP(2M-IKQE)
Plasmid#185757PurposeBacterial expression of MBP-GFP(2M IKQE)DepositorInsertGFP(2M-IKQE)
UseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pT7-eGFP
Plasmid#32552DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterpT7Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB6638
Plasmid#106165PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-GpNLuc
Plasmid#135935PurposeExpresses the GpNLuc LumiFluor in mammalian cells.DepositorInsertGpNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TCF7
Plasmid#79498PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDSDepositorInsertTCF7 (TCF7 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-NC
Plasmid#30226DepositorInsertrhVitronectin with N and C terminus cleavages (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478, 20-62PromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-8x
Plasmid#172284PurposeExpressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequenceDepositorInsertEGFP-N1-8x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12g1
Plasmid#120879PurposeExpresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.DepositorInsertCas12g1
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV MN (eGFP)
Plasmid#89932PurposeExpresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorInsertMN
UseTagsHAExpressionMammalianMutationM.SssI residues 2-272 fragmentPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template1-3XMyc-Spacer
Plasmid#176208PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template1-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas12i-Max-T2A-EGFP
Plasmid#188268PurposeMammalian expression, Genome editingDepositorInsertCas12i-Max
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas12i-HiFi-T2A-EGFP
Plasmid#188269PurposeMammalian expression, Genome editingDepositorInsertCas12i-HiFi
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_minSV40-sfGFP-CMV_dsRed
Plasmid#117400PurposesfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TOP1
Plasmid#183323PurposeAll-in-One CRISPRko system with a guide RNA that targets TOP1 geneDepositorInsertTOP1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CCR5-donor-MCS
Plasmid#192269PurposeDonor template for CRISPR Genome EditingDepositorInsertMCS within donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP_LD 0_TEV
Plasmid#58877PurposeMESA protease chain with Rapamycin FKBP ectodomain and no linker domainDepositorInsertMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
UseAAVTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only