Addgene

We narrowed to 18,528 results for: omp

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Plasmid

#217511

Purpose

KD Recombinase dependent conditional smGdP-10XHA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Use

Tags

smGdP-10XHA

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-PLproCS-MBP

Plasmid

#169195

Purpose

To express a PLpro substrate (GST-PLproCS-MBP) in E. coli

Depositor

John Diffley

Article

Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

Insert

GST-PLproCS-MBP

Use

Tags

GST-MBP fusion (PLpro-cleavable) and His6 (intern…

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

Plasmid

#113949

Purpose

mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 21-852

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO)6-(SIM)10

Plasmid

#122027

Purpose

Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS hygro

Plasmid

#158634

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-IL6.Pro

Plasmid

#79491

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Il6 Promoter (IL6 Synthetic, Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

IL6 Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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Tet-Inducible Human Caspase-9 Alpha

Plasmid

#194708

Purpose

Inducible Expression of Human Caspase-9 Alpha

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Human Caspase-9 Alpha (CASP9 Human)

Use

Tags

None

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTP434

Plasmid

#104167

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)

Use

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

3x Cysteines located at bps 493-495, 520-522, and…

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-MIB1-IRES-Puro

Plasmid

#140240

Purpose

Lentiviral vector expressing flag-tagged MIB1

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Flag-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET41b+_Ufd1-His

Plasmid

#117107

Purpose

Production of recombinant Ufd1-His in E.coli

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

Ufd1 (UFD1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-SpdCas9-W3SL

Plasmid

#210708

Purpose

This Plasmid express hSyn promoter driven SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

hSyn

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-mKdm2b (del CxxC domain)-Flag

Plasmid

#61740

Purpose

Express CxxC domain deleted mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mutation

delete aa 588-624

Promoter

EF1a

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only
hnRNPA2_LC_WT

Plasmid

#98657

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#1-mVenus_PGK-H2B-CFP

Plasmid

#164100

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#1-mVenus

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_BTK-1/1

Plasmid

#91350

Purpose

Protein expression and purification of human SH3 domain construct BTK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BTK-1/1 (BTK Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI1A-3×FLAG

Plasmid

#215509

Purpose

Expresses FLAG tagged human WIPI1A.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep152

Plasmid

#136825

Purpose

Mammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep152 (CEP152 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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Nsp12-EGFP

Plasmid

#165116

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp12 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Fer-SH2

Plasmid

#46425

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fer (FER Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 450-558

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-5HRE-GFP NeoR

Plasmid

#128960

Purpose

Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

5X HRE of VEGF (VEGFA Human)

Use

Lentiviral

Tags

d2EGFP

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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