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Plasmid#44848PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selectionDepositorInsertyoLSS-mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x4
Plasmid#63604PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.sfGFP-RepEntry.iPAC
Plasmid#100893PurposeLentiviral CRISPR-Cas9 ReporterDepositorInsertssfGFP
PAC
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mis12 INCENP Δcen mCherry
Plasmid#45228DepositorInsertMis12 INCENP Δcen mCherry
UseTagsVSVGExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
Plasmid#32474DepositorInsertPSAMQ79G,Q139G:5HT3 HC
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)
Plasmid#101105PurposeEncodes dCas9(C) (without VP64) fused to VEGFR1DepositorInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZLSLD.R
Plasmid#51511PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLS234
Plasmid#73682PurposeSapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable markerDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-myristo
Plasmid#109541PurposeMultisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5DepositorInsert5' myristoylation sequence from ARF5
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM2_4
Plasmid#183057PurposeHAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locusDepositorInsertsmRuby2
HAA1-mTurquoise2
UseSynthetic BiologyTagsmRuby2 and mTurquoise2ExpressionYeastMutationPromoterHAA1 and YGP1Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9–mKate2ps–EgRNA
Plasmid#62716PurposeEmpty; no U6 gRNA cassetteDepositorInsertCas9–mKate2ps
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTORKm43GW
Plasmid#112897PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418).DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGbH500-Nanog-AXP
Plasmid#140279Purposea targeting plasmid for Nanog-APEXDepositorInsertAPEX2
UseMouse TargetingTagsExpressionMutationPromoterendogenousAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHL661
Plasmid#37635DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
NSm-GFP pZK538
Plasmid#118012Purposeexpresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi DNA assembly cloning (NEB).DepositorArticleInsertsNSm
mCherry
UseTagsEGFP and HDELExpressionBacterial and PlantMutationPromoter35SAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHFX-cas3UD
Plasmid#174385PurposePlasmids for gene knockout were constructed based on suicide plasmid pHFXDepositorInsertCas3UD
UseArchaeal gene knockout vector for haloferax medit…TagsExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH3F
Plasmid#85773PurposeN-ICE plasmid pH3FDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC208
Plasmid#70017PurposeTALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl VirusDepositorInsertNuclease (TALENs) + Donor + GVR
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH2B-mPlum/Gag
Plasmid#102255Purposeco-expression of Gag and H2B Plum in mammalian cellsDepositorInsertsGag
H2B Plum
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-ATG18
Plasmid#109556PurposeMultisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2DepositorInsertyeast ATG18 (PLEKHA3)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only