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Plasmid#209012PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(143-421)
Plasmid#209007PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR
Plasmid#200638PurposejGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cellsDepositorInsertjGCaMP7c
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tc-INTR-GFP
Plasmid#208899Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the beetle Tribolium castaneum, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA010.188
Plasmid#206792PurposeJ23113.dCas9DepositorInsertdCas9
UseTagsExpressionMutationPromoterJ23113Available sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK389
Plasmid#206791PurposeSp.dCas9, pTet.MCP-SoxS, J23119.gRNADepositorInsertsdCas9
MCP-SoxS
scRNA
UseTagsExpressionMutationPromoterJ23119 and TetAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT009.119.DA9
Plasmid#206803PurposeExpresses DA9 scRNA for activation of pDA303DepositorInsertDA9 scRNA
UseTagsExpressionMutationPromoterJ23119Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA303.DA9.1B.mRFP
Plasmid#206802PurposemRFP with synthetic promoter HP3 (from Figure S21)DepositorInsertmRFP
UseTagsExpressionMutationPromoterDA9.1B (HP3)Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.156_LV-Zeo
Plasmid#205554PurposeExpress zeocin resistance markerDepositorInsertZeo
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.11_Gag-MCP_ZF2-P2A-eGFP
Plasmid#205526PurposeExpress HIV Gag-MCP_ZF2-P2A-eGFPDepositorInsertGag-MCP_ZF2
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1-A-ptet-dCas9
Plasmid#190129PurposeLevel 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRiDepositorInsertdCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMB1-A-ptet-FLAG-dCas9
Plasmid#190130PurposeLevel 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRiDepositorInsertdCas9
UseCRISPRTagsFLAGExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0019_T2c_BCD_0.1K
Plasmid#200057PurposeBicistronic RBS 0.1K PartDepositorInsertBCD 0.1K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0028_T2c_BCD_18K
Plasmid#200048PurposeBicistronic RBS 18K PartDepositorInsertBCD 18K
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0021_T2c_BCD_0.45K
Plasmid#200055PurposeBicistronic RBS 0.45K PartDepositorInsertBCD 0.45K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0020_T2c_BCD_0.2K
Plasmid#200056PurposeBicistronic RBS 0.2K PartDepositorInsertBCD 0.2K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHY_P0026_T2c_BCD_8.5K
Plasmid#200050PurposeBicistronic RBS 8.5K PartDepositorInsertBCD 8.5K
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
UseTags6His-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg20-24 pST39
Plasmid#200439PurposeHis6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.DepositorInsertsAtg20
Atg24
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only