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Plasmid#18030DepositorInsertWnt-3a (Wnt3a Mouse)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pnxABE-flgn
Plasmid#187932PurposeA-to-G base editorDepositorInsertecTadA(8e)-nxCas9 (D10A)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pconst #4-RFP
Plasmid#81078Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23107Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-PDHA1-N-10
Plasmid#56356PurposeLocalization: Mitochondria, Excitation: 530, Emission: 541DepositorInsertPDHA1 (PDHA1 Human)
UseTagsmPapaya1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1L2
Plasmid#113735PurposeGateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher EQ
Plasmid#35045DepositorInsertSpyCatcher EQ
UseTagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…PromoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lepr peak reporter-eGFP
Plasmid#196584PurposeCis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3DepositorInserteGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-FKBP-U2AF35
Plasmid#187969Purposeexpresses U2AF35 with N-terminal tags RFP and FKBPDepositorInsertU2AF35 (U2af1 Mouse)
UseCmv promoterTagsmRFP-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
UseTagsExpressionBacterialMutationPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Sm
Plasmid#45937Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOtop2_pGEMHE
Plasmid#114675PurposeThis construct was used to express mouse Otop2 in Xenopus laevis oocytesDepositorInsertOtopetrin 2 (Otop2 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationPromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR3
Plasmid#183291PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR3 geneDepositorInsertFGFR3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT29-splitD
Plasmid#122599Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
SauCas9 EMX1-pegRNA
Plasmid#169857PurposeSauCas9 pegRNA for EMX1DepositorInsertSauCas9 EMX1-pegRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]
Plasmid#159896PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
RFP-FKBP-PRPF38A
Plasmid#187968Purposeexpresses PRPF38A with N-terminal tags RFP and FKBPDepositorInsertPrp38A (PRPF38A Synthetic, Human)
UseCmv promoterTagsmRFP-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRNA-U6-let7 sponge
Plasmid#35664DepositorInsertU6 let-7sponge
UseTagsExpressionMutationPromoterU6Available sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDendra2-H2a
Plasmid#157816PurposeFluorescent reporter for histone H2A trackingDepositorInsertH2A (H2ac13 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas7
Plasmid#178879PurposeExpression of human codon-optimized Nlacas7 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas7c-bGH PolyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only