Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 11061 - 11080 of 20580 results

  1. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

    Enlarge

  2. pMMACE DEST CMV SpCas9

    Plasmid
    #206268
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206268-plasmid-map-sequence-id-406404

    Enlarge

  5. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  6. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Availability
    Academic Institutions and Nonprofits only
    62210-plasmid-map-sequence-id-101869

    Enlarge

  7. pDD346

    Plasmid
    #133311
    Purpose
    SapTrap mNG-C1 donor
    Depositor
    Daniel Dickinson
    Insert
    C. elegans codon-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans codon-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    133311-plasmid-map-sequence-id-263341

    Enlarge

  8. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

    Enlarge

  9. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

    Enlarge

  10. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

    Enlarge

  11. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

    Enlarge

  12. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

    Enlarge

  13. pCASCADE-gapAP1

    Plasmid
    #87146
    Purpose
    gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Availability
    Academic Institutions and Nonprofits only
    87146-plasmid-map-sequence-id-174938

    Enlarge

  14. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

    Enlarge

  15. pDD376

    Plasmid
    #137022
    Purpose
    SapTrap mKate2-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized mKate2
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    137022-plasmid-map-sequence-id-274081

    Enlarge

  16. pDD400

    Plasmid
    #137024
    Purpose
    SapTrap mNG-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    137024-plasmid-map-sequence-id-274031

    Enlarge

  17. pMS051

    Plasmid
    #137025
    Purpose
    SapTrap mScarlet-I-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized mScarlet
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    137025-plasmid-map-sequence-id-274042

    Enlarge

  18. pSpCas9(BB)-2A-Neo

    Plasmid
    #127762
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    127762-plasmid-map-sequence-id-247554

    Enlarge

  19. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128323-plasmid-map-sequence-id-255024

    Enlarge

  20. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

    Enlarge

Showing: 11061 - 11080 of 20580 results