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gfp-Arkadia/RNF111

Plasmid

#112228

Purpose

Expresses GFP-tagged Arkadia in mammalian cells

Depositor

Vasso Episkopou

Publication

Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

(Arkadia)Rnf111 (Rnf111 Mouse)

Tags

GFP

Expression

Mammalian

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB-EEA-5g-OSK2M2L1-PGK-Puro

Plasmid

#102909

Purpose

PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GG-EBNA-OSK2M2L1-PP

Plasmid

#102902

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OSK2M2L1-gRNAs-PGK-puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.CAG.SF-iGluSnFR.A184V

Plasmid

#106199

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLU-CMV-Flag-hATG5 WT

Plasmid

#111803

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Publication

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Archon1-KGC-EGFP-ER2

Plasmid

#108420

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-SAC1-FKBP

Plasmid

#108129

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Publication

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)

Expression

Mammalian

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRIPZ Myc2-mouse TTP WT

Plasmid

#107000

Purpose

Expresses epitope-tagged wild-type murine TTP

Depositor

Julian Downward

Publication

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (Zfp36 Mouse)

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.CAG.SF-Venus-iGluSnFR.A184S

Plasmid

#106201

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-9myc-AID*(N)

Plasmid

#99527

Purpose

N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA5

Plasmid

#97040

Purpose

Expresses HOXA5 in mammalian cells

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A5 (HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Publication

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
AKARet-cyto

Plasmid

#96897

Purpose

FLIM sensor for PKA activation in cytosol

Depositor

Ryohei Yasuda

Publication

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLIM Sensor for PKA Activity in Cytosol

Tags

EGFP and sREAChet

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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8027_GFP-KrasG12C_2B_puro

Plasmid

#64372

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Publication

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_MAX_WT_V5

Plasmid

#82944

Purpose

Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_EGFR_p.T790M_p.L858R

Plasmid

#82914

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

T790M, L858R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p{CFD4-3xP3::DsRed}

Plasmid

#86864

Purpose

Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1a-FLuc-WPRE-HGHpA

Plasmid

#87951

Purpose

Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

Depositor

Mark Kay

Publication

Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

Insert

Firefly Luciferase

Use

AAV

Promoter

EF1a

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSLI-sandwich(0807600)

Plasmid

#85790

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

Depositor

Tobias Spielmann

Publication

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R

Use

P. falciparum genomic integration

Tags

2xFKBP, GFP

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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