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Viral prep#198511-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarlet (Cre-dependent)Available sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only
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pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRExpressionMammalianAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IVT-donor_opt(R2Tg)-GFP
Plasmid#223246PurposeIn vitro transcription of the optimized R2Tg RNA donorDepositorInsertR2Tg donor_opt
UseIn vitro transcriptionAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
IVT-R2Tg_opt
Plasmid#223247PurposeIn vitro transcription of the optimized R2Tg proteinDepositorInsertR2Tg protein_opt
UseIn vitro transcriptionAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ABCB1_STOP
Plasmid#221424PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertABCB1 (ABCB1 Human)
ExpressionMammalianAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-EcSTH-FLAG
Plasmid#218628PurposeThis plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.DepositorInsertEcSTH
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PCNA
Plasmid#215117PurposeExpresses mCherry-PCNA in mammalian cells from a lentiviral vector.DepositorAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-EF1a-i53
Plasmid#216243PurposeExpress i53 in mammalian cells as a HDR enhancerDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserti53
TagsFlagExpressionMammalianPromoterEF1aAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE (AAV5)
Viral prep#162374-AAV5PurposeReady-to-use AAV5 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR
Plasmid#210523PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA-directcapture
UseAAVExpressionMammalianAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-mitoGshF-blasticidin
Plasmid#210314PurposeExpresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cellsDepositorInsertBifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
UseRetroviralTagsFLAG peptide, Homo sapiens ACO2 mitochondrial tar…MutationCodon optimized for expression in human cellsAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-8
Plasmid#208786PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorInsertCaspase 8 (CASP8 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH624-ssr
Plasmid#199241PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertsingle-stranded DNA recombineering
ExpressionBacterialPromoterlacIq-PtrcAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PE-Max-pet21A
Plasmid#204471PurposeExpression plasmid for PE protein in bacteria.DepositorInsertPrime editor
UseCRISPRTagsHis-tagExpressionBacterialAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AXBEv2
Plasmid#204606PurposeMammalian expression of evolved adenine transversion base editor AXBEv2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)Available sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TFORF3358
Plasmid#144834PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-AsCas12f-sgRNA-v2
Plasmid#204639PurposeExpression of trancated AsCas12f sgRNA in mammalian cellsDepositorInserttrancated AsCas12f sgRNA (sgRNA-v2)
ExpressionMammalianPromoterU6Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDH-WT-ALKBH5-3xFLAG
Plasmid#203736PurposeOverexpression of ALKBH5-WTDepositorAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLexM-SLC3A2
Plasmid#197994PurposeExpresses SLC3A2 as an N-terminal His tagged construct in mammalian cellsDepositorInsertSLC3A2 (SLC3A2 Human)
TagsHis tagExpressionMammalianMutationPlease see depositor comments belowPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only