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L40C-CRISPR.EFS.mNeon

Plasmid

#69146

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl

Plasmid

#90485

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKD239-1g-2

Plasmid

#90959

Purpose

Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23109, and PttrB185-269

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pKD238-1a

Plasmid

#90958

Purpose

Expresses S. baltica TtrS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Expression

Bacterial

Promoter

Bba_J23109

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-3xHA-DHFR-LoxP

Plasmid

#86669

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-3xHA-HXGPRT 3'UTR

Use

Expression in toxoplasma gondii

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B T23N

Plasmid

#89450

Purpose

Expression of GFP-tagged human Rab27B T23N in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B T23N (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

T23N

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-PhoCl-mCherry-myc

Plasmid

#87692

Purpose

Express PhoCl-mCherry with C-term myc tag in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl-mCherry-myc

Tags

myc tag

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TP53_WT

Plasmid

#82754

Purpose

Gateway Donor vector containing TP53, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TP53 (TP53 Human)

Use

Gateway Cloning

Mutation

P72R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAi14-GFPNLS-MC

Plasmid

#87114

Purpose

minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFPNLSPpA

Use

CRISPR

Promoter

nEF

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pKSCTMF

Plasmid

#85073

Purpose

Gene insertion/deletion in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi

Insert

AOX1promoter - mazF - AOX1 tt- Zeocin R gene

Use

Gene insertion/deletion in pichia pastoris

Promoter

pAOX1

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

Plasmid

#85070

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Tags

IRES-sYFP2-PH and mScarlet-H

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pY036_ATP1A1_G5_Array

Plasmid

#86620

Purpose

Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA

Use

CRISPR; Co-selection via hdr using ouabain

Tags

3xHA

Expression

Mammalian

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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SIVh

Plasmid

#81091

Purpose

Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM5-mSHMT2

Plasmid

#83905

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 2 (Shmt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-ZnT1-WT

Plasmid

#82340

Purpose

Expresses FLAG-tagged ZnT1-WT in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

ZnT1-WT (SLC30A1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP43

Plasmid

#78953

Purpose

Figure 2. Expresses BxbI.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

BxbI

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9

Plasmid

#80425

Purpose

Used for in vitro transcription of SpCas9 mRNA.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9

Use

CRISPR

Tags

none

Mutation

codon optimized for human

Promoter

T7

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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