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pBIG1d

Plasmid

#80614

Purpose

pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB550

Plasmid

#74880

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Publication

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Expression

Yeast

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

Plasmid

#153008

Purpose

Expresses glucagon peptide on mammalian cell surface

Depositor

Alice Ting

Publication

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Glucagon-ICAM1-pre-mGRASP

Tags

3x HA

Expression

Mammalian

Promoter

CAG

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirAonly

Plasmid

#232584

Purpose

Express HA-BirA

Depositor

Sagar Bhogaraju

Publication

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BirA

Tags

HA

Expression

Mammalian

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMyc0ElbLuc

Plasmid

#53242

Purpose

reporter plasmid without myc cDNA binding sites

Depositor

Roger Davis

Publication

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pMycOElbLuc (MYC Human)

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoPA-TagRFP-SpHis5

Plasmid

#44854

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-TagRFP

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-link-yomKeima-Kan

Plasmid

#44902

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Expression

Yeast

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pRS303 Gal TDP-43 YFP

Plasmid

#27468

Depositor

Aaron Gitler

Publication

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

D23A mutation in TDP-43 sequence

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPE-10u

Plasmid

#13542

Depositor

Wolf Frommer

Publication

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLIP-E A207R

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207R in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-BAI3

Plasmid

#116716

Purpose

Lentiviral expression of BAI3

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BAI3 (BAI3 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAC1-P29S

Plasmid

#116650

Purpose

Lentiviral expression of RAC1 P29S

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAC1 (RAC1 Human)

Use

Lentiviral

Mutation

P29S

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAG413GPD-ccdB-EGFP

Plasmid

#14190

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro

Plasmid

#248161

Purpose

Expresses KRAB fused to dCas9 and BFP upon cumate induction

Depositor

Joanna Wysocka

Publication

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

KRAB-dCas9-HA-tagBFP
HA-CymR-T2A-puro

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Promoter

Ef1a

Available since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pNP3386 [For use in genome insertion and off-target assessment]

Plasmid

#247258

Purpose

Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

Depositor

Patrick Hsu

Publication

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])

Expression

Mammalian

Available since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNP2390 [pNP2322 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

Plasmid

#247252

Purpose

Measure ISCro4 activity via mCherry expression

Depositor

Patrick Hsu

Publication

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2322 - ISCro4_Donor, ISCro4_Target_RTG-Matched

Expression

Mammalian

Available since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-mouse-Nb-Hia5

Plasmid

#239626

Purpose

Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Publication

Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Mouse Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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