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pET30a-SARS-CoV2-NP

Plasmid

#165090

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)

Expression

Bacterial

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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ACE2-Fc

Plasmid

#164222

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

Depositor

Sriram Neelamegham

Publication

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 extracellular domain (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Publication

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLA1

Plasmid

#160807

Purpose

Express POLA1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLA1 (POLA1 Human)

Use

Retroviral

Mutation

Codon Optimized

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe_Zeo_cOVA_T2A_mStrawberry

Plasmid

#161736

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Publication

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL9

Plasmid

#149520

Purpose

Express APEX2-Galectin-9 in mammalian cells

Depositor

Vojo Deretic

Publication

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LGALS9 (LGALS9 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-PH-TAPP1

Plasmid

#161985

Purpose

Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

Depositor

Dario Alessi, David Bryant

Publication

Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PH-TAPP1 (PLEKHA1 Human)

Tags

GFP

Expression

Mammalian

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mNeonGreen-MapTau

Plasmid

#137808

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MapTau

Expression

Mammalian

Promoter

CMV

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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JD638

Plasmid

#160395

Purpose

Vitis miR396-resistant GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Publication

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis miR396-resistant GRF4-GIF1 chimera

Use

Entry clon

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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JD630

Plasmid

#160394

Purpose

Vitis GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Publication

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis GRF4-GIF1 chimera

Use

Entry clon

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Publication

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMXs_FLAG-NMNAT2

Plasmid

#133260

Purpose

Express FLAG-tagged NMNAT2 in mammalian cells

Depositor

David Sabatini

Publication

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT2 (NMNAT2 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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6 x DBE-luc

Plasmid

#153160

Purpose

Luciferase reporter plasmid for FOXTF

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

luciferase

Use

Luciferase

Promoter

minP

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-BiPOLES-mCerulean

Plasmid

#154950

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Publication

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-EYFP (AAV PHP.V1)

Viral prep

#104052-AAVPHP.V1

Purpose

Ready-to-use AAV PHP.V1 particles produced from pAAV-CAG-DIO-EYFP (#104052). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-EYFP plasmid DNA. CAG-driven, Cre-dependent expression of EYFP. These AAV were produced with the PHP.V1 serotype, which permits efficient transduction of brain vascular cells. These AAV preparations are suitable purity for injection into animals.

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Promoter

CAG

Tags

EYFP (Cre-dependent)

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126706

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Publication

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)

Use

CRISPR

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFP-VP64-GB1

Plasmid

#141417

Purpose

Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

Depositor

Izuho Hatada

Publication

Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

scFvGCN4sfGFP-VP64-GB1

Tags

HA and sfGFP

Expression

Mammalian

Promoter

CAG

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-XI-Neo-GST-Constitutively Active AMPK

Plasmid

#139843

Purpose

Lentiviral expression of GST-tagged Constitutively Active rat AMPK

Depositor

Jacob Corn

Publication

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-puro/RAF1

Plasmid

#131725

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Publication

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRRL-GFPAdGO-PGK-Neo

Plasmid

#136898

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Publication

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPAdGO

Use

Lentiviral

Tags

2xNLS

Mutation

TGG>TGA

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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