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Twist-siRNA5

Plasmid

#8456

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
Twist-siRNA7

Plasmid

#8457

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoSuperfolderGFP-SpHis5

Plasmid

#44837

Purpose

Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoSuperfolderGFP

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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Cdc2

Plasmid

#1886

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pACU2

Plasmid

#31223

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Type

Empty backbone

Expression

Insect

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra2

Plasmid

#179475

Purpose

Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra2
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mClover3

Plasmid

#133453

Purpose

Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mClover3

Tags

GSGSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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SpyTag003-Talin rod-mCherry

Plasmid

#133567

Purpose

Expresses SpyTag003-Talin rod-mCherry in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-Talin rod-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flp-DOG-NW

Plasmid

#75469

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Flp-DOG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-ephrinB1-SV40-RFP (b1R)

Plasmid

#65446

Purpose

Expression of ephrinB1 and RFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

ephrin B1 (Efnb1 Mouse)

Use

Lentiviral

Tags

SV40-RFP

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-flbio

Plasmid

#30216

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

No gene

Use

Gateway Cloning

Tags

Bio and Flag

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pCXN2KS-Dsup

Plasmid

#90019

Purpose

To establish stable line expressing Dsup in constitutive manner

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Expression

Mammalian

Promoter

CAG

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HisC EXO1b

Plasmid

#68268

Purpose

Mammalian expression of human EXO1

Depositor

Stefano Ferrari

Article

El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

Insert

EXO1 (EXO1 Human)

Tags

His

Expression

Mammalian

Mutation

b form, codons 1-846. Please see depositor commen…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pFPV25

Plasmid

#20667

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Tags

GFP mut3

Expression

Bacterial

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pLX - T7pro - EBFP

Plasmid

#138615

Purpose

Express EBFP with an upstream T7 promoter in a lentiviral backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EBFP

Use

Lentiviral

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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JWW-2 human chimeric monoclonal antibody

Plasmid

#66749

Purpose

Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-2 Heavy Chain
JWW-2 Light Chain

Expression

Mammalian

Promoter

mEF1 and rEF1

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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JWW-1 human chimeric antibody

Plasmid

#66748

Purpose

Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa

Expression

Mammalian

Mutation

First 3 amino acids of rat light chain constant r…

Promoter

mEF1 and rEF1

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAGM9121

Plasmid

#51833

Purpose

level 0 Universal acceptor

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

March 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pClneoFH human RASSF1A

Plasmid

#37016

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

rassf1A (RASSF1 Human)

Tags

FLAG and His

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-CFP-SaCas9-HF (without sgRNA)

Plasmid

#134470

Purpose

Human expression vector for SaCas9-HF variant.

Depositor

Zongli Zheng

Article

Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

Insert

Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity

Use

CRISPR

Tags

SV40NLS-3xFLAG

Expression

Mammalian

Mutation

R245A, N413A, N419A, R654A

Promoter

CAG

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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