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154,674 results

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pYPQ132-tRNA

Plasmid

#179211

Purpose

Golden Gate entry vector; 2nd gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

no promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm4b-pA

Plasmid

#104486

Purpose

Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

Depositor

Yi Zhang

Article

Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

Insert

Kdm4b (Kdm4b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2.1-Ago1

Plasmid

#22306

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

AGO1

Use

2micron

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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p1321 HPV-16 E6/E7

Plasmid

#8641

Depositor

Peter Howley

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV-16 E6/E7 (E7, E6 Human papillomavirus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9a

Plasmid

#41721

Purpose

Retroviral expression of human G9a in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9a (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A-NG

Plasmid

#220895

Purpose

Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

Plasmid

#223273

Purpose

Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

Plasmid

#63931

Purpose

Empty AAV-RAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

pRAM and TRE

Available since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

AtU3d

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Article

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Use

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-FOXO4

Plasmid

#153143

Purpose

Expression of Flag tagged human FOXO4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXO4 (FOXO4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCT-tRNA

Plasmid

#133813

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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p23-NES-RfxCas13d-msfGFP-NES-Flag

Plasmid

#165076

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEXTK-Pak1 70-117

Plasmid

#12217

Depositor

Jonathan Chernoff

Insert

Pak1 (PAK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

PBD (p21-binding domain), aa 70-117

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pLD-puro-MTS-EGFP

Plasmid

#190270

Purpose

Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

Depositor

Christian Münch

Article

Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

Insert

MTS-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Tight TRE promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 Gag-imCherry

Plasmid

#208882

Purpose

codes for Gag from HIV-1 with an internal mCherry fluorescent protein

Depositor

Raphael Gaudin

Article

Diana Brychka et al BioRxiv

Insert

HIV-1 Gag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MARCer-Blast

Plasmid

#160550

Purpose

labeling hypoxic cells

Depositor

Marc Vooijs

Article

Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

Insert

MARCer (HIF1A Human)

Use

Lentiviral

Tags

eGFP-Cre

Expression

Mutation

Promoter

CMV

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Robert Malinow, Andres Barria

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

Plasmid

#105842

Purpose

Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
Optimized mApple
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

CAG, EF-1alpha, and TRE3G

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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