Addgene

We narrowed to 17,881 results for: puromycin

Showing: 1081 - 1100 of 17881 results

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pPyPGK-EGFP-TM-IRES-Pac

Plasmid

#183604

Purpose

Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

Human PDGFRB transmembrane domain and Mouse IgGK …

Expression

Mammalian

Mutation

Promoter

Mouse Pgk1

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P26

Plasmid

#120037

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 S22N

Plasmid

#107106

Purpose

Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196079

Purpose

Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA

Plasmid

#130912

Purpose

Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG-IRES2-mNeonGreen

Plasmid

#175506

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G-mCMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFH2.155_LV-Puro-T2A-mTagBFP2

Plasmid

#205553

Purpose

Express puromycin resistance marker and mTagBFP2

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Puro-T2A-mTagBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P29

Plasmid

#120040

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P11

Plasmid

#120022

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pscALPS SIV-MND2 vpx

Plasmid

#115811

Purpose

Encodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMND2 Vpx (AY159322)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMY-eGFP-P2A-PuroR-HA

Plasmid

#163343

Purpose

Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-mCherry-P2A-PuroR-HA

Plasmid

#163342

Purpose

Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV Tet

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P25

Plasmid

#120036

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-CTR1-shRNA

Plasmid

#53180

Purpose

Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
L40C-CRISPR.EFS.PAC

Plasmid

#89393

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM-D4-miR30-TRIM5

Plasmid

#132932

Purpose

Lentivector for TRIM5 knockdown and puromycin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

TRIM5 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-RCM vpx

Plasmid

#115812

Purpose

Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM Vpx (AF349680)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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