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Plasmid#188972PurposeFor red light-repressed bacterial expression of gene of interest (StrR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only
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pX330A_FokI-1x2
Plasmid#63602PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
bbCas9pluspAAA
Plasmid#82581PurposeThe plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNADepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPaCas9
Plasmid#111819PurposeExpresses Cas9 in T. brucei (2T1) cellsDepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x4
Plasmid#63598PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cHAIAA17
Plasmid#48990Purposefor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsIAA17 degron tagExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x7
Plasmid#63601PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x4
Plasmid#63604PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x7
Plasmid#63607PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x6
Plasmid#63606PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x3
Plasmid#63603PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-spo5DSR
Plasmid#49867Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJUMP38-1A(sfGFP)
Plasmid#126984PurposeLevel 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x5
Plasmid#63605PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-6XDSR
Plasmid#49865Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-3XDSR
Plasmid#49863Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-spo5DSR
Plasmid#49872Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-6XDSR
Plasmid#49870Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-3XDSR
Plasmid#49868Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-8XDSR
Plasmid#49866Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only