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Plasmid#83877PurposeDestination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assaysDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only
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L0-I39 PhiC31-Intein GG1-GG4
Plasmid#195905PurposeLevel 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkersDepositorInsertPhiC31-intein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I40 Intein-PhiC31 GG1-GG4
Plasmid#195906PurposeLevel 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkersDepositorInsertIntein-PhiC31
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VC_Cas1_WT
Plasmid#120364PurposeExpresses wildtype V-C Cas1DepositorInsertVC_Cas1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3a-His-MBP-TEV-VD_Cas1_WT
Plasmid#120365PurposeExpresses wildtype V-D Cas1DepositorInsertVD_Cas1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR1
Plasmid#120366PurposePlasmid containing shortened version of upstream V-C CRISPR array for integration assaysDepositorInsertCRISPR1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-CRISPR
Plasmid#120369PurposePlasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing libraryDepositorInsertsCRISPR1
CRISPR2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD_CRISPR
Plasmid#120370PurposePlasmid containing shortened version of V-D CRISPR array for integration assaysDepositorInsertVD_CRISPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNL2080 pGATA23 GG0-GG1
Plasmid#195908PurposeLevel 0 pGATA23 promoter with GG0 and GG1 golden gate linkersDepositorInsertpGATA23
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSHS293
Plasmid#87348PurposeD435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/D435C/C574S/E945CPromoterT7Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)
Plasmid#39300DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationS16A, R17A, H18A, D19A, W23A and N25APromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Rca_Cas13a_WT
Plasmid#91870PurposeBacterial expression for Cas13aDepositorInsertRcaCas13a (RcaC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA1-lenti
Plasmid#115662PurposeExpresses AcrVA1 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA1
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPP384
Plasmid#158799PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-3 (D413A)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Cam_Cas13a_WT
Plasmid#91869PurposeBacterial expression for Cas13aDepositorInsertCamCas13a (CamC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Ere_Cas13a_WT
Plasmid#91868PurposeBacterial expression for Cas13aDepositorInsertEreCas13a (EreC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-AFB2
Plasmid#83845PurposeGateway donor vector for AFB2DepositorInsertAFB2 (AFB2 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
7xNLS-pMJ915v2-sfGFP
Plasmid#88922PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti
Plasmid#138318PurposeLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFPDepositorInsertSauCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only