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Addgene
Showing: 1081 - 1100 of 16046 results

  3. pDONR221-deltaUBA-short-UBASH3A-Ctag

    Plasmid
    #195397
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195397-plasmid-map-sequence-id-388659

    Enlarge

  4. pDONR221-deltaUBA-short-UBASH3A-Ntag

    Plasmid
    #195398
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195398-plasmid-map-sequence-id-386315

    Enlarge

  6. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176575
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    176575-plasmid-map-sequence-id-348273

    Enlarge

  7. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175788
    Purpose
    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Use
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    175788-plasmid-map-sequence-id-345285

    Enlarge

  9. pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #175801
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Use
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    175801-plasmid-map-sequence-id-345278

    Enlarge

  10. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  11. HOXA5 (human) HIS-tag pET

    Plasmid
    #8562
    Depositor
    Corey Largman
    Insert
    HOXA5 (HOXA5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    SacI site at amino acids 1-2 was used to clone in…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  12. HOXC5 (human) HIS-tag pET

    Plasmid
    #8563
    Depositor
    Corey Largman
    Insert
    HOXC5 (HOXC5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    Starts at Amino Acid #2 (Ser); makes protein by W…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. HOXC8 (murine) HIS-tag pET

    Plasmid
    #8567
    Depositor
    Corey Largman
    Insert
    HOXC8 (Hoxc8 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  19. HOXA1 (murine) HIS-tag pET

    Plasmid
    #8556
    Depositor
    Corey Largman
    Insert
    HOXA1 (Hoxa1 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    clone contains 130 AA including the homeodomain, …
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  20. HOXA2 (murine) HIS-tag pET

    Plasmid
    #8557
    Depositor
    Corey Largman
    Insert
    HOXA2 (Hoxa2 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    contains 5 additional amino acids from 5' un…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1081 - 1100 of 16046 results