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Plasmid#185917PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)
Plasmid#197507PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)
Plasmid#197502PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)
Plasmid#197508PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…PromoterCMV and T7Available sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-sgR26-eCas9-T2A-BlastR
Plasmid#172493PurposeFor CRISPR-assisted HDR, Rosa26 locusDepositorInserteCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)
Plasmid#197504PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-ABE8e-NRCH-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ad:2CMV_A2R2-N25Ctr
Plasmid#122243PurposeExpresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backboneDepositorUseAdenoviralTagsSignal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…ExpressionMammalianMutationdeleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…PromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)
Plasmid#197499PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)
Plasmid#197505PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…PromoterCMV and T7Available sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CYLBL_Neo_CAGdCas9-KRAB
Plasmid#220439PurposeCAG-dCas9-KRAB knock-in using TALEN at CLYBL locusDepositorInsertdCas9
UseCRISPR and TALENTagsKRABExpressionMammalianMutationD10A and H840APromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN
Plasmid#172435PurposeLentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKLDepositorInsertsCre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)
Plasmid#197501PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)
Plasmid#197500PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
XE92 XGCNF-EnR
Plasmid#17029DepositorInsertXGCNF-EnR (en Fly)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP
Pooled Library#153101PurposeCRISPR library targets all non-essential and conditionally essential transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp1
Pooled Library#153102PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp2
Pooled Library#153103PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp3
Pooled Library#153104PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp4
Pooled Library#153105PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sctrans-PP-Grp5
Pooled Library#153106PurposeCRISPR library targets a sub-pool of transporter genes in S. cerevisiaeDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only