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Plasmid#214921Purposeconstitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylationDepositorInsertSRRD (SRRD Human)
UseLentiviralTagsV5-APEX2ExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c022
Plasmid#175490PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.DepositorInsertSYK (SYK Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C - AVITEV
Plasmid#74058Purposeretroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hH4-AVITEV
Plasmid#74051Purposeretroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman Histone-H4 (H4C16 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC - AVITEV
Plasmid#74059Purposeretroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-bio-myc-mCE 2-210
Plasmid#112715PurposeExpresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsbiotinylation signal peptide (Tagwerker et al. 20…ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMVAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-bio-myc-mCE 211-597
Plasmid#112716PurposeExpresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsbiotinylation signal peptide (Tagwerker et al. 20…ExpressionMammalianMutationdeleted amino acids 2-210 (triphosphatase domain)PromoterCMVAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44-isoform1-CD4d3+4-bio
Plasmid#73098PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform1 with rat CD4d3+4-bioDepositorInsertCD44 antigen isoform 1 (CD44 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD59-CD4d3+4-bio
Plasmid#73117PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD59 with rat CD4d3+4-bioDepositorUseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD44-isoform12-CD4d3+4-bio
Plasmid#73130PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform12 with rat CD4d3+4-bioDepositorInsertCD44 antigen isoform 12 (CD44 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a HsDHFR-bio-10His
Plasmid#193883PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequenceDepositorInserthuman dihydrofolate reductase (DHFR) (DHFR Human)
UseTagsHis tag and biotinylation sequenceExpressionBacterialMutationCodon optimizedPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(1-686)AVITEV
Plasmid#74054Purposeretroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIG-AVITEV- hNFATc1/aA
Plasmid#74057Purposeretroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform alpha-A (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationmutation G1157A [R235Q] and the silent mutation C…PromoterpMSCV-LTRsAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
Plasmid#74053Purposeretroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-bio-myc-cCE
Plasmid#112711PurposeFor tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cellsDepositorInsertRngtt (Rngtt Mouse)
UseTagsHIV Rev nuclear localization signal (NES), Stop c…ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMV with Tet repressor binding sitesAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV
Plasmid#74055Purposeretroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNICBio2_WDR5:2-334
Plasmid#211027Purposebacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.DepositorInsertWDR5:A2-C334 (WDR5 Human)
UseTagsMHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationwild typePromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_WDR12:90-422
Plasmid#210893PurposeInsect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR12:T90-G422 (WDR12 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_WDR41:30-459
Plasmid#210892PurposeInsect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR41:Q30-V459 (WDR41 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only