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Plasmid#154365PurposeYeast expression of Nup124DepositorInsertNup124 (nup124 Fission Yeast)
UseTagsExpressionYeastMutationSee depositor comments belowPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cherry-SRBC
Plasmid#69492PurposeExpression of fluorescently tagged SRBC in mammalian cellsDepositorInsertSRBC (Cavin3 Mouse)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Triple ORF FRT ifgMosaic Donor (IG135)
Plasmid#99626PurposeTo clone the 3 ORFs for FlpO dependent generation of ifgMosaicsDepositorInsertPGK-Neo
UseCre/Lox, Mouse Targeting, and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT2-H2B-EGFP-V5-2A (IG157)
Plasmid#99623PurposeTo clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassetteDepositorInsertH2B-EGFP-V5
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A1-VN
Plasmid#135502PurposeMammalian expression of VN-fused and myc-tagged HLA-A*01:01DepositorInsertHLA-A*01:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-135
Plasmid#31067DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-135 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b1-Poly(A)-NeoR
Plasmid#65551PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-NHL only-LIN-41
Plasmid#52723Purposeretroviral expression of human LIN-41 / TRIM71 containing only the NHL repeats and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationNHL-only contains only an initiating methionine a…PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-7CtoA-LIN-41
Plasmid#52726Purposeretroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged cysteines 12, 15, 61, 66, 69, 91, and 94 …PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO-tet-puro-sh-ex16b
Plasmid#35168DepositorInsertFoxp1 (Foxp1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
FRT3-Mb2-HA-mTfp1-2A (IG155)
Plasmid#99621PurposeTo clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2ADepositorInsertMb2-HA-mTfp1
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT1-Mb2YFP-2A (IG153)
Plasmid#99619PurposeTo clone gene of interest downstream of FRT1-Mb2YFP-2A cassetteDepositorInsertMb2EYFP
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT2-Mb2Tomato-2A (IG154)
Plasmid#99620PurposeTo clone gene of interest downstream of FRT2-Mb2Tomato-2A cassetteDepositorInsertMb2Tomato
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT3-HA-H2B-Cerulean-2A (IG158))
Plasmid#99624PurposeTo clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassetteDepositorInsertHA-H2B-Cerulean
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-2B-Nkx2-5 delta HD-G8
Plasmid#98614PurposeGateway entry vector NKX2-5 _HDDepositorInsertNKX2-5 _HD (Nkx2-5 Mouse)
UseEntry vectorTagsExpressionMutationDeletion of the homeodomain. Replaced by 8 glycin…PromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 E17K;K177M
Plasmid#73049PurposeHuman AKT3 E17K;K177MDepositorInsertAKT3 E17K;K177M (AKT3 Human)
UseTagsExpressionMammalianMutationE17K;K177MPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-226
Plasmid#31064DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-226 to -1 of P2 HNF4A promoterPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Keratin-17
Plasmid#57629PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertKeratin (KRT18 Human)
UseTagstdEosExpressionMammalianMutationV282LPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
FRIG-myc-Sema4D-CD4
Plasmid#51606Purposeexpresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backboneDepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseLentiviralTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterRSVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-K74A K75A
Plasmid#49253PurposeMammalian expression of flag-tagged human Med26-K74A K75ADepositorInsertMed 26 (MED26 Human)
UseTagsHAExpressionMammalianMutationK74A K75APromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only