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Plasmid#91035PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertTaCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC440
Plasmid#91225Purposeprotoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC441
Plasmid#91218Purposeprotoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC436
Plasmid#91222Purposeprotoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC437
Plasmid#91219Purposeprotoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ATF3
Plasmid#67057PurposeStandard for cell-free expression benchmarking.DepositorInsertATF3 (ATF3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationM2IPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTC394
Plasmid#91224Purposeprotoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAH750
Plasmid#91212Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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gabra5_R (TAL3015)
Plasmid#36007DepositorInsertTAL array targeting gabra5 (gabra5 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
gabra5_L (TAL3014)
Plasmid#36006DepositorInsertTAL array targeting gabra5 (gabra5 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pAAVf-EnhCB-lacZnls miR-122 3x
Plasmid#35644DepositorInsert3 miR-122 target sites
UseAAVTagsExpressionMutationPromoterCBAvailable sinceApril 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)
Plasmid#60245PurposeExpress the RBD of mouse MSI1 in bacterial cells for protein purificationDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains aa7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CALR
Plasmid#67086PurposeStandard for cell-free expression benchmarking.DepositorInsertCALR (CALR Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only