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Plasmid#60220PurposeMammalian expression of FLAG-tagged catalytic mutant of TUT4DepositorInsertTUT4 (Tut4 Mouse)
UseTagsFLAGExpressionMammalianMutationD1028APromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CAG-RORb2-IRES-GFPd2
Plasmid#73998PurposePlasmid expressing RORb2-IRES-GFPd2DepositorInsertRORb2 ORF (Rorb Mouse)
UseTagsnoneExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x5
Plasmid#58775PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-HJURP-CBP
Plasmid#30393DepositorInsertHJURP CenpA Binding Domain (CENPA Human, Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV TBG FFluc miR122sponge
Plasmid#35657DepositorInsertmiR-122 sponge
UseAAVTagsExpressionMutationPromoterTBGAvailable sinceJuly 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTD-C_eYFPTwinStrep
Plasmid#45942Purposeexpresses E.coli codon optimized EYFP-Twin-Strep fusion proteinDepositorInsertsynthetic eYFP (codon usage adapted to E.coli K12)
UseTagsTwin-Strep-tagExpressionBacterialMutationPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXylA-RgTALsyn
Plasmid#100948PurposeExpression of Tyrosine Ammonia Lyase from constitutive XylA promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterXylA (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v3
Plasmid#79539PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 3DepositorInsertAmrose v3
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUSE-RapR-Src(KD)-myc
Plasmid#25934DepositorInsertRapR-Src (Fkbp1a Mouse)
UseTagsExpressionMammalianMutationThis pUSE-RapR-Src(KD)-myc plasmid contains the k…PromoterAvailable sinceOct. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-gp160
Plasmid#123273PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)DepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutationsPromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mEGFP/FRT/TO
Plasmid#27762DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SIMx6
Plasmid#116938PurposeBacterial expression vector for 6 simDepositorInsert6 sim
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SUMOx10
Plasmid#116937PurposeBacterial expression vector for 10 sumoDepositorInsert10 SUMO
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1710
Plasmid#91039PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 NFKB1
Plasmid#67126PurposeStandard for cell-free expression benchmarking.DepositorInsertNFKB1 (NFKB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNMT3b KO Hyg
Plasmid#16650DepositorInsertDNMT3b knock-out construct (DNMT3B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(APY)
Plasmid#15742DepositorInsertJnk1a1(TPF) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationThr-190AlaPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTC364
Plasmid#91213Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -