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Plasmid#159524PurposeExpresses mCherry from LAP2 promoterDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterLAP2Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEn-Chimera
Plasmid#61432PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backboneDepositorInsertAtU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-SIRT6
Plasmid#102325PurposeHuman SIRT6 for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
SMN2E7-Fluc-Nluc-reporter
Plasmid#221005PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.DepositorInsertFluc-SMN2E7-STOP-Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-MHC-IIA
Plasmid#35687DepositorInsertMHC IIA (MYH9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-134/150TAG
Plasmid#174077PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationD134TAG + N150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-H2BGFP
Plasmid#26790DepositorInsertH2B-GFP (H2BC21 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
Piggybac-TetO-hNurr1-blast
Plasmid#176485PurposeTetO-hNurr1 in a PiggyBac vector with Blasticidin resistanceDepositorInsertNurr1 (NR4A2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-anti-AlfaTag nanobody
Plasmid#171819PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInserttdTomato-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TurboRFP-pBAD
Plasmid#54858PurposeLocalization: Protein Expression Vector , Excitation: 553, Emission: 574DepositorTypeEmpty backboneUseTags6xHis and TurboRFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mem
Plasmid#55779PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.DepositorInsertmCherry-Mem
UseTagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-mWnt1
Plasmid#176486Purposemouse Wnt1 cDNA in TetO backboneDepositorInsertmouse Wnt1 cDNA (Wnt1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only