Addgene

154,649 results

Showing: 10801 - 10820 of 154649 results

Results per page:

Sort results by:

nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p1321 HPV-16 E6/E7

Plasmid

#8641

Depositor

Peter Howley

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV-16 E6/E7 (E7, E6 Human papillomavirus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-RUNX1

Plasmid

#192928

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX1 (RUNX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23a-H6-TEV-cSrc

Plasmid

#214233

Purpose

Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

Depositor

Neel Shah

Article

Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

Insert

cSrc Kinase domain (SRC Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNICKclos2.0

Plasmid

#73228

Purpose

Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sgRNA to xylR

Use

E.coli - clostridium shuttle vector

Tags

Expression

Mutation

D10A

Promoter

Pj23119 and Pthl

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
IKK-2 WT

Plasmid

#11103

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS tdTomato

Plasmid

#91767

Purpose

Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

tandem dimer Tomato

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Gsdmd-NT

Plasmid

#80951

Purpose

Expresses Gsdmd-NT in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT (Gsdmd Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-TRE3G-NLS-tdPCP-EGFP

Plasmid

#99910

Purpose

For the expression of NLS-tPMCP-EGFP

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-tdPCP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE4G

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-EGFP_Hu_p115-RhoGEF(WT)

Plasmid

#136337

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV_Cas9_puro

Plasmid

#65655

Purpose

Retroviral introduction of Cas9 into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Insert

Cas9

Use

CRISPR and Retroviral

Tags

5 ' FLAG tag

Expression

Mammalian

Mutation

Promoter

MSSV_LTR

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcVpx.myc

Plasmid

#213388

Purpose

Generate lentiviral vector which increases gene delivery into primary human myeloid cells

Depositor

Nathaniel Landau

Article

Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

Insert

SIVmac vpx (vpx Simian Immunodeficiency Virus)

Use

Lentiviral

Tags

myc-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi_RP_MicroID2-HA

Plasmid

#188521

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

Plasmid

#61594

Purpose

A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt4

Plasmid

#35910

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC-4.06

Plasmid

#112084

Purpose

NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)

Depositor

James Inglese

Article

Baird et al Elife. 2018 Mar 12;7. pii: 33178. doi: 1

Insert

3XFLAG firefly luciferase-beta-globin 39PTC (HBB Photinus pyralis, Human, Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge