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Showing: 10781 - 10800 of 24771 results

  1. PX458 eSp(1.1) V3

    Plasmid
    #226963
    Purpose
    CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226963-plasmid-map-sequence-id-450822

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  6. pDule2-Acd82

    Plasmid
    #174100
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174100-plasmid-map-sequence-id-345717

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  8. pFastBac HT JS-Rab10wt

    Plasmid
    #135557
    Purpose
    Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.
    Depositor
    Jingshi Shen
    Article
    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11
    Insert
    Rab10 (Rab10 Mouse)
    Use
    Tags
    6xHis tag, TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135557-plasmid-map-sequence-id-272501

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  12. MF81_PB-TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid
    #176625
    Purpose
    TF A self-activation cassette in MultiFate-2.1
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-12x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    176625-plasmid-map-sequence-id-454352

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  16. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid
    #227245
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227245-plasmid-map-sequence-id-450568

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Showing: 10781 - 10800 of 24771 results