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Plasmid#183096PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nspGCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpGCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-VP64
Plasmid#50918PurposeConstitutive dCas9-VP64 lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCNA
Plasmid#135191PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-CreI.DepositorInsertI-CreI lamba Red
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoteraraBADAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP3A4
Plasmid#67104PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP3A4 (CYP3A4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-iRFP670-Tdtomato-GFP
Plasmid#111817PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsiRFP670
Tdtomato
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-eGFP
Plasmid#32548DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBLO1811_arC9_noNLS_human
Plasmid#74494PurposeHuman codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNADepositorInsertarC9
UseCRISPRTagst2a mCherry (C-terminal on insert)ExpressionMammalianMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
LZRS-hNEDD9_IresGFP
Plasmid#21962DepositorInsertNEDD9 (NEDD9 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
hCas9-VPR
Plasmid#68497Purposenuclease competent SP-Cas9 fused to VPRDepositorInsertSP-Cas9-VPR
UseTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3-Neo-EF1a-GFP
Plasmid#69134PurposeSleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promotersDepositorTypeEmpty backboneUseSleeping beauty transposonTagsExpressionMammalianMutationPromoterU3MSCVAvailable sinceJan. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-CaMKIIA
Plasmid#186825PurposeExpresses FLAG-CaMKIIA in mammalian cellsDepositorInsertCamk2a (Camk2a Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0208 CMV-TO-BFPmut-stop-t70587 (FLP-IN)
Plasmid#191138PurposeExpression of Trigger 1 in 3' UTR of an inactive BFP in mammalian cellsDepositorInsertt70587
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_YB TATA-sfGFP-CMV_dsRed
Plasmid#117399PurposesfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Spy EMX1-pegRNA
Plasmid#169855PurposeSpyCas9-pegRNA for EMX1DepositorInsertSpy EMX1-pegRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-GFP-Bub1-wt
Plasmid#16624DepositorInsertBUB1 (BUB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (no Cre)
Plasmid#200370PurposeBackbone plasmid for cloning HiUGE GS-gRNA (without Cre)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT pentamutant for Split-PE (pEH107)
Plasmid#190110PurposeExpresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationD14R, N26R, D74R, N116K, N197RPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -