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Plasmid#42205DepositorInsertc-SRC (1-397) (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 1-397PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-mTET1_CD
Plasmid#83571PurposeExpresses a mutant catalytic domain of TET1 in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseTags3X FLAG tag and hr GFP IIExpressionMammalianMutationTET1 amino acids 1-1417 deleted, catalytic domain…PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (91-536)
Plasmid#42206DepositorInsertc-SRC (91-536) (SRC Human)
UseTagsExpressionMammalianMutationcontains amino acids 91-536PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE
Plasmid#127864PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 48-232 (#182)
Plasmid#99920Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationN-terminal deletion; encodes AA 48-232PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(D)
Plasmid#87914PurposeExpresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.DepositorInsertFmr1 (Fmr1 Mouse)
UseTagsFlag and eGFPExpressionMammalianMutationS499DPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 77-232 (#426)
Plasmid#99921Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationN-terminal deletion; encodes AA 77-232PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-CTRLg1
Plasmid#139450PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest iRFP
Plasmid#62176PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf37
Plasmid#12725PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf37
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf48
Plasmid#12736PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf48
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1 for LuxR, pLux for GFPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM4788
Plasmid#120089PurposeCyanobacteria synthetic plasmid harboring the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS) to test the pSYSA-TA1 toxin–antitoxin cassette.DepositorInsertpAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rec-PhoA/CsgA
Plasmid#170787PurposeRecombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinaseDepositorUseSynthetic BiologyTagsHHHHHHExpressionBacterialMutationPromoterInverted proD with Bxb1recognition sites and Inve…Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IRES-hrGFP-mWnt5a
Plasmid#89772PurposeExpresses mouse Wnt5a in mammalian cellsDepositorInsertmouse Wnt5a (Wnt5a Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NES
Plasmid#175008PurposeCytosol-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PHD3
Plasmid#21402DepositorInsertEGLN3 (EGLN3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg1 (CG509)
Plasmid#139455PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NMYC-mTRF1
Plasmid#64162PurposeRetroviral vector expressing mouse TRF1 with N-terminal Myc tagDepositorInsertmTRF1 (Terf1 Mouse)
UseRetroviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only