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Plasmid#115824PurposeEncodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance proteinDepositorInsertSIVASC Vpr (KJ461715)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-DEB vpr
Plasmid#115823PurposeEncodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance proteinDepositorInsertSIVDEB Vpr (FJ919724)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-LST vpr
Plasmid#115818PurposeEncodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance proteinDepositorInsertSIVLST Vpr (AF188116)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-MND1 vpr
Plasmid#115817PurposeEncodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance proteinDepositorInsertSIVMND1 Vpr (M27470)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMY-mTurquoise2-P2A-PuroR-HA
Plasmid#163344PurposeRetroviral vector to express cytosolic mTurquoise2 and an HA-tagged puromycin selection markerDepositorInsertmTurquoise2
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-COL-BWC01 vpr
Plasmid#115840PurposeEncodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance proteinDepositorInsertSIVCOLVpr (KF214240)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-RCM-vpr
Plasmid#115838PurposeEncodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance proteinDepositorInsertSIVRCM Vpr (HM803689)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-SUN-K08 vpr
Plasmid#115832PurposeEncodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance proteinDepositorInsertSIVSUN Vpr (FR751162)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-GSN-CN166 vpr
Plasmid#115831PurposeEncodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance proteinDepositorInsertSIVGSN-CN166 Vpr (AF468659)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-SYK vpr
Plasmid#115821PurposeEncodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance proteinDepositorInsertSIVSYK Vpr (L06042)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-TAL vpr
Plasmid#115820PurposeEncodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance proteinDepositorInsertSIVTAL Vpr (AM182197)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P30
Plasmid#120041PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-GDepositorInsertVSV-G
UsePcr templateTagsVSV-GExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-AGI vpx
Plasmid#115815PurposeEncodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance proteinDepositorInsertSIVAGI Vpx (HM803690)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-DRL vpx
Plasmid#115814PurposeEncodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance proteinDepositorInsertSIVDRL Vpx (KM378563)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-MNE vpx
Plasmid#115813PurposeEncodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance proteinDepositorInsertSIVMNE Vpx (U79412)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Sa-gRNA-puro
Plasmid#191652PurposeLentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.DepositorInsertS. aureus gRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-tetO-mCherry-attB53
Plasmid#183612PurposeRMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInserttetO-mCherry
UseRmceTagsExpressionMutationPromotertetOAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.wt IRES Puro
Plasmid#110387Purposeγ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only