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Plasmid#199369PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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CRISPRainbow Kit for Multiplex Labeling
Plasmid Kit#1000000079PurposeCRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.DepositorAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-DreO-bGHpA (AAV5)
Viral Prep#50363-AAV5PurposeReady-to-use AAV5 particles produced from AAV phSyn1(S)-DreO-bGHpA (#50363). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-DreO-bGHpA plasmid DNA. hSyn-driven Dre recombinase expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-DreO-bGHpA (AAV Retrograde)
Viral Prep#50363-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV phSyn1(S)-DreO-bGHpA (#50363). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-DreO-bGHpA plasmid DNA. hSyn-driven Dre recombinase expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b-T4gp45
Plasmid#228204PurposeExpression in BL21(DE3) of WT T4 clampDepositorInsert45 (45 Escherichia phage T4)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaryP
Plasmid#18945DepositorInsertphiC31 attP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJF3H
Plasmid#63897PurposeFor phage display of cDNA librariesDepositorInsertcDNA cloning region
UsePhage displayTagsFosExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4-4T-1 BCP1 NTase
Plasmid#161812PurposeExpresses SMODS/CD-NTase from Bacillus phage Bcp1DepositorInsertphage BCP1 SMODS/CD-NTase enzyme
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTA-attP
Plasmid#18939DepositorInsertphiC31 attP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pRVV601
Plasmid#87632PurposeRMCE Bxb1 attB-flanked vector.DepositorInsertBxb1 attB
UseRmceTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVV651
Plasmid#87631PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-sfGFP
Plasmid#51562PurposeExpression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)DepositorInsertsuperfolder GFP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVV647
Plasmid#87630PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVV653
Plasmid#89474PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVV598
Plasmid#87629PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only