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MSCV-(pbabe puro mcs)-human CSF-1 full length (CSF4)-IRES-GFP

Plasmid

#86796

Purpose

retroviral expression of human full length CSF-1(256)

Depositor

Charles Sherr, Martine Roussel

Article

Heard et al Oncogene Res. 1987 Sep-Oct;1(4):423-40.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHub10-His-YFP-PIP2;1

Plasmid

#99386

Purpose

overexpression and isolation of recombinant At-PIP2;1 protein

Depositor

Ning Li

Article

Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

Insert

PIP2;1 (PIP2A Mustard Weed)

Use

Tags

His and YFP

Expression

Plant

Mutation

Promoter

2xCaMV35S

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA,3Q)

Plasmid

#192444

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(WT)

Plasmid

#27134

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-tdTomato-f

Plasmid

#127092

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tdTomato-f

Use

AAV

Tags

farnesylation signal from c-Ha-Ras

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-Y2404F

Plasmid

#182846

Purpose

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Use

Tags

Expression

Bacterial

Mutation

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pShuttle-EGFP-CLASP2 340-1362 9xS/A

Plasmid

#24373

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAdEasy-EGFP-CLASP2 340-1084 9xS/A

Plasmid

#24525

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) 9xS/A (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pShuttle-EGFP-CLASP2 340-1084 9xS/A

Plasmid

#24384

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Expression

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pUT_mNF-Cas13d-Triplex

Plasmid

#227709

Purpose

LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

mCherry-P2A-RfxCas13d-Triplex

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV-d2i

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-G2304D

Plasmid

#182845

Purpose

mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

mutation G2304D (GGC/GAC)

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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RAPpGEX

Plasmid

#162241

Purpose

Expresses LDL-receptor associated protein (RAP), full length in mammalian cells

Depositor

Andreas Faissner

Article

Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45

Insert

lrpap1 (Lrpap1 Mouse)

Use

Tags

GST-tag

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCasper4-g-osk-TRICK

Plasmid

#71244

Purpose

oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

oskar (osk Fly)

Use

P element-mediated germline transformation

Tags

12xPP7 and 6xMS2

Expression

Insect

Mutation

Promoter

osk

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2-YFP-CWT

Plasmid

#176689

Purpose

Fluorescent reporter for phagosomal escape of Staphylococcus aureus

Depositor

Martin Fraunholz

Article

Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

Insert

cell wall-targeting domain of Lysostaphin

Use

Tags

YFP

Expression

Mammalian

Mutation

deletion of protase domain of Lysostaphin

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA,3Q)-NLS

Plasmid

#192445

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-NFLAG-hFUS

Plasmid

#50487

Purpose

Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

TRE

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
ECON

Plasmid

#82553

Purpose

Epigenetic effector construct for targeted methylation

Depositor

Joseph Irudayaraj

Article

Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)

Use

Tags

HALO and mCherry

Expression

Mammalian

Mutation

N-terminal photolyase homology region domain of C…

Promoter

CMV

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-CLASP2 512-650 9xS/A

Plasmid

#24410

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant that c…

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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