Addgene

We narrowed to 2,325 results for: gem

Showing: 1061 - 1080 of 2325 results

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rs769-minor

Plasmid

#113364

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs7697634 minor allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs7697634 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs769-major

Plasmid

#113363

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs7697634 major allele (C)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs7697634 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs622-minor

Plasmid

#113360

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs62288111 minor allele (C)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs62288111 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs622-major

Plasmid

#113359

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs62288111 major allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs62288111 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs795-minor

Plasmid

#113356

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs7957274 minor allele (C)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs7957274 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs795-major

Plasmid

#113355

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs7957274 major allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs7957274 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs699-major

Plasmid

#113357

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs6990531 major allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs6990531 major allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCas59

Plasmid

#82396

Purpose

sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-scFv-T

Plasmid

#67843

Purpose

pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

Depositor

Joanna Bereta

Article

Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

Insert

antibody 1D8 light kappa chain

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-Cyclin-B

Plasmid

#177012

Purpose

Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

Cyclin B1 (CCNB1 Human)

Use

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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TOM20MTS-mCherry-EGFP-Tet-On

Plasmid

#109016

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

TOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PMP34-mCherry-EGFP-Tet-On

Plasmid

#109017

Purpose

Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

PMP34 (SLC25A17 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-h53BP1 (siRNA resistant)

Plasmid

#110301

Purpose

Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GAACGAGGA to GAGCGGGGC

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA331Bb

Plasmid

#78269

Purpose

pSEVA331 with Biobrick multiple cloning site

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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