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Plasmid#197506PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)
Plasmid#185916PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-sgH11-eCas9-T2A-BlastR
Plasmid#172492PurposeFor CRISPR-assisted HDR, Hipp11 locusDepositorInsert, BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)
Plasmid#185915PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
R26-dCas9-VPR-mNG
Plasmid#172441PurposeRosa26 targeting vector, expresses dCas9-VPR and mNeonGreenDepositorInsertsdCas9-VPR
mNeonGreen
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-sgfiller-eCas9-T2A-BlastR
Plasmid#172437PurposeExpresses eCas9, BlastR and sgRNA cloning siteDepositorInsertseCas9
BlastR
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ad:2CMV_A2R2-N25K3
Plasmid#122242PurposeExpresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backboneDepositorUseAdenoviralTagsSignal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV-PGK-Cre-EFS-mScarletSIIN
Plasmid#172434PurposeLentivirus, expresses Cre recombinase and mScarlet-SIINFEKLDepositorInsertsCre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(TARDBP)
Plasmid#109323PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBPDepositorInsertmCherry-KASH (TARDBP Human)
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-crRNA(SOD1)
Plasmid#109321PurposeAAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1DepositorInsertmCherry-KASH (SOD1 Human)
UseAAVTagsExpressionMutationPromoterhSyn1Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MK01
Bacterial Strain#195090PurposeImproved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).DepositorBacterial ResistanceChloramphenicolAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN
Plasmid#172436PurposeLentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning siteDepositorInsertsCre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)
Plasmid#185912PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
Plasmid#197511PurposeCbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacerDepositorInsertAAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
UseAAV and CRISPRTagsBPNLS and BPNLS-NpuC(intein)ExpressionMutationC-terminal mutations of SpRY(L1111R/D1135L/S1136W…PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)
Plasmid#197503PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT1-2A-eGFP PGK PuroR Donor Plasmid
Plasmid#82333PurposeWT1 targeting donorDepositorUseCRISPR and TALENTags2A-eGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)
Plasmid#197509PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpCas9(D10A)PromoterCbhAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only