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Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
UseTags6xhis-tag and MBPExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag
Plasmid#109030PurposeWild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(His10-PD-1cyto)
Plasmid#177896PurposeE coli expression of His10 tag fused to human PD-1 Intracellular DomainDepositorInsertPD-1 (aa 194-288 only) (PDCD1 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 His10-FcRɣ
Plasmid#113021Purposefor purification of His10-FcRɣ intracellular domainDepositorInsertHis10-FcRɣ intracellular domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hPKM2
Plasmid#42515DepositorInsertPyruvate Kinase M2 (PKM Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24b-6His-fSNAP
Plasmid#106999PurposeTo express 6His-fSNAP protein in E.coliDepositorInsertfSNAP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-myc
Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and mycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbPpep-CHis
Plasmid#48243PurposeFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domainDepositorInsertClbP-pep
UseTags6-HisExpressionBacterialMutationFirst 375 amino acids of the peptidase, which con…PromoterT7Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Ets1-ETS
Plasmid#85735PurposeExpresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440DepositorInsertDNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Human, Mouse)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del2 pET28a
Plasmid#122116PurposeExpression Brg1 amino acids 1-837 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTags6XHis and His-T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986H
Plasmid#174801PurposeBacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overallDepositorInsertPARP1-Y986H (PARP1 Human)
UseTags6xHisExpressionBacterialMutationY986H, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986S
Plasmid#174802PurposeBacterial expression of a PARP1 mutant that produces mainly short PAR oligomersDepositorInsertPARP1-Y986S (PARP1 Human)
UseTags6xHisExpressionBacterialMutationY986H, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G972R
Plasmid#174800PurposeBacterial expression of a PARP1 mutant that produces less PAR oligomers overallDepositorInsertPARP1-G972R (PARP1 Human)
UseTags6xHisExpressionBacterialMutationG972R, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG-F6C
Plasmid#176546PurposeExpresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange phenylalanine 6 to cysteine; change glutam…PromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA
Plasmid#40236DepositorInsertLOV-ipaA
UseTags6xHisExpressionBacterialMutationJα helix region hybridized (AsLOV2 aa 537-546/ipa…PromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only