Addgene

We narrowed to 1,350 results for: MiCy

Showing: 1061 - 1080 of 1350 results

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pCRISPR2_IHF_Mut

Plasmid

#149396

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHFprox site is mutated at key positions needed f…

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pmRE-Tn5-142

Plasmid

#118531

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-gamma-tubulin-TEV-His6

Plasmid

#178067

Purpose

Insect cell expression of gamma-tubulin-TEV-His6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin (TUBG1 Human)

Use

Tags

TEV-His6

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDON207 NSP3 K977Q

Plasmid

#180057

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 K977Q (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 K977Q

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON207 NSP3 A890D

Plasmid

#180069

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 A890D (ORF1ab SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 A890D

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Use

Tags

GST tag and His tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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DYNLL2

Plasmid

#132531

Purpose

Human LC8-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL2 (DYNLL2 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLT3

Plasmid

#132532

Purpose

Human TCTEX-3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT3 (DYNLT3 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG SMAD4

Plasmid

#83094

Purpose

Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

SMAD4 (SMAD4 Human)

Use

Entry vector for gateway cloning

Tags

3xFLAG

Expression

Mutation

Promoter

TRE tight promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pACEBAC1-CoREST-C

Plasmid

#109159

Purpose

Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Use

Tags

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Bjoern Sander, Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Bjoern Sander, Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(WT)

Plasmid

#86576

Purpose

Expresses cleavable human full-length Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-Gm129-Flag

Plasmid

#53766

Purpose

baculovirus expression of Gm129

Depositor

Aziz Sancar

Article

Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

Insert

Gm129 (Ciart Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pCas3cRh

Plasmid

#133773

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Joseph Bondy-Denomy

Article

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8
Cas7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAcBac1.tR4-AcdRS82

Plasmid

#174101

Purpose

Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

M. barkeri tRNA synthetase for acridone82

Use

Tags

Expression

Mammalian

Mutation

N311S, C313G, V366A, W382T

Promoter

CMV

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Use

Tags

Strep Sumo

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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